摘要
作者研究了影响油菜对毒素草酸抗性的一些因素以及运用毒素草酸法筛选抗菌核病材料的效果。结果表明:油菜苗用草酸浸根后,苗病害严重度分别随温度、草酸浓度和草酸处理时间的增加而增大,随湿度、苗龄的增大而减小。在用草酸法鉴定品种的抗性时,草酸浓度10～15mmol/L,温度18～28℃,相对湿度80％～90％和自然光照对2～5片真叶期苗适合。用草酸浸根和浸叶法筛选油菜抗病材料的效果明显。用草酸浸叶法筛选的8个品种(系),其单株后代群体田间自然侵染鉴定的病情指数较原群体(未作筛选的自由授粉)平均减小65.6％,第2代自由授粉群体降低33.5％。第2代群体对草酸的抗性比原群体平均提高13.9％。经草酸浸根法筛选后的苗再接种病菌,其病斑面积较原群体减小1.7％～68％。
Factors affecting resistance of rape cultivars to oxalic acid (OA) and the efficiency of using OA to screen resistance rape to Sclerotinia sclerotiorum was investigated. The results showed that toxic index increased along with temperature and OA concentration , respectively. It decreased with the increase of relative humidity and seedling age, respectively. The change of toxic index with treatment time followed Logistic-growth curve. The optimal conditions for the test of cultivar resistance using OA were studied. Eight cultivars (lines) were selected by soaking detached leaves. The disease index of the progeny populations of the selected single plant decreased by 65. 6% compared to non-selected natural pollinated population. The disease index of the natural pollinated populations of the following generation reduced 33. 5%. The diseased leaf area of root treated seedlings reduced 1.7% -68% compared to that of the original population.
出处
《植物保护学报》
CAS
CSCD
北大核心
1998年第1期56-60,共5页
Journal of Plant Protection
基金
国家自然科学基金
"八五"国家攻关
关键词
核盘菌
草酸
油菜
抗性筛选
油料作物
Sclerotinia sclerotiorum, oxalic acid, oilseed rape, resistant screening methods
