摘要
本研究探讨了培养基种类、酸碱度、温度对菌核菌(Sclerotinia sclerotiorum)菌丝生长(MG)和草酸累积(OA)的影响及MG和OA之间的动态变化过程。正交试验中,碳源为:琥珀酸钠-葡萄糖、蔗糖-马铃薯汁液和蔗糖的三种培养基中,OA量依次递减且差异显著。MG和OA成负相关关系。培养基的pH值是影响OA的一个主要因子,本试验中pH6最适合菌丝产草酸,而pH5最适合菌丝生长。23℃下OA和MG大于26℃下OA和MG。OA和MG的动态变化过程试验表明,不同培养基的草酸累积速率和最终量差异显著。本文认为碳源种类或草酸前体物是影响OA的主要因子。
The effects of various media, pH, temperature and time course on mycelial growth (MG) of and oxalic acid accumulation (OA) by Sclerotinia sclerotiorum in the culture were studied. It was shown in an orthogonal experiment design (L_(16)4~3×2~6) that OA were significantly different (p<0.05) when glucose succinate, sucrose potato juice and sucrose were used as carbon source in the media. The medium containing glucose succinate was most effective for OA. However, MG was negative correlation with OA across different media. The initial pH values of media were a primary factor in regulating OA. Optimum pH for OA was 6, but optimum pH for MG was 5. OA and MG at 23℃ were more than those at 26℃. In a separate study by using both the medium containing glucose succinate and the medium containing sucrose it was found the rate of OA and final amount, of OA were significantly different (p<0.05) between the two media. These results suggested that amount of precursor of oxalic acid (carbon source easily metabolized to oxalic acid is called as precursor of oxalic acid) was a main factor affecting OA.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第4期196-199,共4页
Microbiology China
关键词
核盘菌
菌丝
生长
草酸
营养
环境
Sclerotinia sclerotiorum
Oxalic acid accumulation
Mycelial growth
Nutritional and Environmental Factors
