摘要
目的:探讨川芎嗪预处理对大鼠移植肝缺血再灌注损伤的作用及可能的机制。方法:将125只雄性SD大鼠随机分成3组:假手术组、对照组(生理盐水作为肝脏灌注液)、实验组(川芎嗪预处理,生理盐水作为肝脏灌注液)。假手术组仅结扎肝动脉,对照组和实验组行大鼠原位肝移植,分别于术后1、6、24、72h剖杀各组大鼠各5只,检测各组大鼠血浆丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量以及血清内毒素水平;检测肝脏组织肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1β)的含量,观察大鼠肝脏组织病理学和Kuffer's细胞超微结构改变。结果:实验组大鼠的ALT、AST、TNF-α、IL-1β的表达明显低于对照组,但高于假手术组(P<0.05);实验组炎症反应及Kuffer's细胞活跃程度明显轻于对照组。结论:川芎嗪预处理能抑制kuffer's细胞吞噬活性,减轻大鼠移植肝缺血再灌注损伤。
Objective：To explore the effection and mechanism of ischemia-reperfusion injury alleviation after performing liver transplantation on rats by pretreatment with ligustrazine. Methods：125 male SD rats were divided into 3 groups：sham operation group,control group（saline used as perfusate）,experimental group（pretreatment with ligustrazine and saline used as perfusate）. The rats in control group and experimental group were performed orthotopic liver transplantation. The levels of serum ALT,AST,TNF-α,IL-1β and endotoxin were detected at 1,6,24,72 h after operation. Liver pathological changes was measured simultaneously. Results：the levels of serum ALT,AST,TNF-α,IL-1β and endotoxin detected in experimental group were lower than those in control group,however higher than those in sham operation group（P 〈 0.05）. Light microscopy result indicated that the inflammation and activation of Kuffer＇s cells in experimental group were less severe than those in control group. Conclusion：Pretreatment with Ligustrazine could protect liver grafts against ischemia-reperfusion injury after liver transplantation in rats.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期733-736,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅135工程资助
