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脑卒中后抑郁大鼠海马齿状回5-羟色胺1A受体的表达 被引量：15

Expression of 5-hydroxytryptamine 1A receptor protein and message RNA in the dentate gyrus in post-stroke depression rats
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摘要 目的观察脑卒中后抑郁（PSD）大鼠海马齿状回5-羟色胺1A（5-HT1A）受体的表达。方法将SD雄性大鼠分为正常对照组、卒中组、应激抑郁对照组和PSD组，每组6只。应用左侧大脑中动脉阻塞（MCAO）联合不可预见的慢性温和应激（CMS）刺激及孤养法建立PSD大鼠模型，采用荧光实时定量聚合酶链反应和Western印迹法检测并比较各组大鼠CMS第19天和第28天齿状回5-HT1A受体（mRNA）和蛋白表达水平。结果（1）CMS第19天，PSD组5-HT1A受体mRNA表达（0．012±0．001）低于正常对照组（0．361±0．010）和卒中组（0．039±0．002；P〈0．001）；其5-HT1A受体蛋白表达（0．400±0．030）低于正常组（1．320±0．060）和卒中组（0．610±0．060；均P〈0．001）。（2）CMS第28天，PSD组5-HT1A受体mRNA（0．013±0．001）低于正常对照组（0．336±0．011）、卒中组（0．063±0．006；均P〈0．001）；其5-HT1A受体蛋白表达（0．080±0．020）低于正常组（0．620±0．030）、卒中组（0．260±0．040）和应激抑郁组（0．320±0．020；均P〈0．001）。结论PSD大鼠海马齿状回5-HT1A受体表达水平降低，此改变可能是PSD发病的分子机制之一。 Objective To investigate the expression of 5-HT1A receptor protein and message Ribonucleic Acid （mRNA） in the dentate gyrus in post-stroke depression （PSD） rats. Methods All 36 male SD rats were divided into 4 groups： control, stroke, depression and PSD group. The PSD model was made by consistent focal cerebral infarcts through left middle cerebral artery occlusion （MCAO）, followed by 18-day exposure to chronic mild stress （CMS） and single housing. The expression of the 5-HT1A receptors protein and mRNA were assayed with Western blotting and real-time reverse-transcription polymerase chain reaction （PCR） on the days 19 and 28 after CMS procedure beginning respectively. Results At day 19, the PSD model rats showed decreased expression of 5-HT1A receptor protein （0. 400 ± 0. 030 vs. 1. 320 ± 0. 060 ; 0.610±0.060, all P〈0.001） and mRNA （0.012±0.001 vs. 0.361±0.010; 0.039±0.002; P〈 0.001, respectively） in comparison with the control and stroke ones respectively. At day 28, the PSD rats showed declined expression of 5-HT1A receptors （0. 080±0. 020 vs. 0. 620±0. 030 ; 0. 260± 0. 040; 0. 320±0. 020, all P 〈0. 001 ） compared to the control, stroke and depressive ones respectively, and lower mRNA （0. 013±0. 001 vs. 0. 336±0. 011 ; 0. 063±0. 006, all P 〈0. 001 ） compared to the control, stroke ones. Conclusion The declined expression of 5-HT1A receptors mRNA and protein in the dentate gyrus in PSD model rats suggests it may be one of the molecular mechanisms in PSD incidence.
作者 王少华 张志珺 郭怡菁 滕皋军 陈宝安
机构地区 东南大学附属中大医院神经内科 分子影像研究中心 东南大学附属中大医院血液内科
出处 《中华精神科杂志》 CAS CSCD 北大核心 2008年第2期107-110,共4页 Chinese Journal of Psychiatry
基金 国家自然科学基金资助项目（30570656 30600206） 江苏省自然科学基金重点资助项目（BK2006708）
关键词 脑血管意外 抑郁 受体 血清素 5-HT1A 海马 齿状回 Cerebrovascular accident Depression Receptor, serotonin, 5-HT1A Hippoeampus Dentate gyrus
分类号 R686 [医药卫生—骨科学]
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参考文献12

  • 1Whyte EM, Mulsant BH. Post stroke depression: epidemiology, pathophysiology, and biological treatment. Biol Psychiatry, 2002, 52:253 -264.
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二级参考文献8

  • 1Willner P. Animal models of depression: an overview. Trends Pharmacol Sci, 1991,12: 131-136.
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  • 8唐启盛,裴清华,侯秀娟,曲淼.脑卒中后抑郁状态动物模型的建立[J].北京中医药大学学报,2004,27(3):33-36. 被引量：51

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中华精神科杂志
2008年 第2期

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