摘要
目的建立脑卒中后抑郁(PSD)动物模型并评价西酞普兰干预作用。方法大脑中动脉阻塞法建立局灶脑缺血模型,加用慢性不可预见温和应激结合孤养,建立 PSD 大鼠模型并予以西酞普兰干预。结果 CMS 开始后第19天,模型大鼠水平活动得分(与基线、正常、卒中组相比均P<0.001;与应激抑郁组相比均 P<0.01),垂直活动得分(与基线、正常、卒中组相比均 P<0.001;与应激抑郁组相比均 P<0.05)显著降低。19～42 d,模型组大鼠水平得分(从37.8±1.7至34.2±1.2)和垂直得分(从9.7±0.8至7.8±0.8)显示与应激抑郁组水平得分(从41.0±1.3至42.3±0.8)、垂直得分(从11.2±0.4至11.7±0.8)程度不同的进行性下降趋势。第3～6周蔗糖水饮用比例显著少于基线及各组(均 P<0.001)。结论模型大鼠充分并持续表现快感缺乏等 PSD 核心症状,可操作性和重复性较好,是研究 PSD 的理想模型。西酞普兰能改善 PSD 大鼠行为学异常。
Objective To establish an animal model of post-stroke depression （PSD） and assess the effects of citalopram on PSD. Methods Fifty-two male SD rats were randomly divided into 3 groups. Normal group （ n = 6） underwent sham operation. Chronic unpredictable mild stress （CMS） group （ n = 6 ）, underwent separate raising and CMS. Middle cerebral artery occlusion （MCAO） group （n = 24） underwent MCAO and then re-divided into 3 equal subgroups： stroke subgroup, PSD model subgroup （undergoing isolated raising and CMS so as to establish PSD models ）, and citalopram subgroup （treated with intraperituneal injection of citalopram, a selective serotonin reuptake inhibitor for 6 weeks）. The rats were examined dynamically at the 19th, 28th, and 42nd days after CMS by open-field test （OFT） and sucrose consumption test. Results On the 19th day after CMS the locomotor activity score of the PSD models was significantly lower （ P 〈0. 001 compared to the baseline level of the same subgroup and to those of the control and stroke rats, P 〈 0.01 compared to the CMS group） ; and the frequency of rearing score of the PSD models was significantly decreased （P 〈 0. 001 compared to its baseline level and to those of the control and stroke groups, and P 〈 0. 05 compared to the CMS group）. During the days 19-42, the frequency of locomotor and exploratory activities of the PSD model rats declined continually, from 37.8 ± 1.7 to 34. 2 ± 1.2 and from 9. 7 ± 0. 8 to 7.8 ± 0. 8 respectively, showing a tendency in just the reverse direction in the CMS group （from 41.0 ± 1.3 to 42. 3±0. 8 and from 11.2±0. 4 to 11.7±0. 8 respectively）. The PSD rats showed a reduction in sucrose preference at week 3 and afterwards （P 〈0. 001 compared with other groups）. The levels of open-field activities and proportion of rats with sucrose preference in the citalopram subgroup were all significantly higher than those in other groups （ all P 〈 0. 001 ） . Conclusion Anhedonia and underaetivity, the core symptoms in the PSD patients, can be found completely and persistently in the PSD rats. With good operability and repeatability, the PSD rat model is an ideal model for PSD research. Citalopram can improve the behavior abnormality in PSD.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第19期1355-1357,共3页
National Medical Journal of China
基金
国家自然科学基金(30570656
30600206)
江苏省自然科学基金重点资助项目(BK2006708)
关键词
脑血管意外
抑郁症
模型
动物
西酞普兰
Cerebrovascular accident
Depressive disorder
Models, animal
Citalopram
