摘要
坐骨神经分支选择性损伤 (SNI)模型是一种新型神经病理痛模型。本实验用 SD雄性大鼠 ,分支结扎并切断左侧坐骨神经干的胫神经和腓总神经 ,保留腓肠神经分支 ,右侧仅暴露坐骨神经。术后 1、2、3、4、7、14、2 1和 2 8d,用 RT-PCR的方法对 5 -HT1 A受体 m RNA在腰髓的背角和背根神经节 (DRG)的表达水平进行检测。结果显示 ,5 -HT1 A受体 m RNA在损伤侧腰髓背角内的表达水平于 1d后开始升高 ,7d时达高峰 ,随后逐渐下降 ,但仍高于正常水平。其表达水平在对侧脊髓背角内没有明显变化。在损伤侧 DRG内 ,5 -HT1 A受体 m RNA的表达水平于 1d后开始增高 ,4d时达高峰 ,随后开始下降 ,但仍维持较高的表达水平 ;而损伤对侧 DRG内的 5 -HT1 A受体 m RNA的表达没有变化。上述结果提示 5 -HT1 A受体亚型可能在脊髓及外周伤害性信息的传递和调节中发挥着重要作用 ,本研究的结果为进一步了解 5 -HT1 A受体在神经病理性痛中的作用机制提供了依据。
By using the reverse transcriptase polymerase chain reaction(RT-PCR) technique, the expression of 5-hydroxytryptamine(5-HT) 1A receptor subtype mRNA in the rat lumbar spinal dorsal horn and dorsal root ganglion (DRG) was investigated in the spared nerve injury (SNI) model. The results showed that 5-HT 1A receptor subtype was present in the rat lumbar spinal dorsal horn and DRG. In the lumbar spinal dorsal horn, the expression level of 5-HT 1A receptor subtype mRNA was observed to increase from 1 day after injury on ipsilateral side, reached a peak at the 7th day. The mRNA level then decreased but was still higher than normal level at 14, 21, 28 days. On contralateral side, the mRNA level of 5-HT 1A receptor subtype remained unchanged. In the DRG, the mRNA level of 5-HT 1A receptor subtype increased from 1 day after surgery on ipsilateral side, reached a maximal level at the 4th day, and then gradually decreased but was still higher than normal level during the observation time windows. No such changes were observed on the contralateral side. The present results indicated the important roles for 5-HT 1A receptor subtype in nociceptive signaling and modulation at both peripheral and spinal cord levels, and provided evidence to elucidate the mechanism of 5-HT 1A receptor subtype in neuropathic pain.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2004年第4期350-354,共5页
Chinese Journal of Neuroanatomy
基金
国家重点基础研究规划"脑功能和重大疾病的基础研究"(G19990 5 40 0 4)
国家自然科学基金 (No. 3 0 2 70 43 8)资助项目
