摘要
目的探讨Ki67和P53蛋白表达在人喉癌发生、发展及预后中的作用。方法采用免疫组织化学Envision法检测182例喉不同病变组织标本(喉癌141例,喉癌前病变18例,声带息肉15例和癌周正常喉组织8例)中Ki67和P53蛋白的表达。结果Ki67、P53在人喉癌组织中表达阳性率均显著高于喉癌前病变、声带息肉组织及癌周正常喉组织(P﹤0.01);中晚期(Ⅲ～Ⅳ期)、伴颈淋巴结转移及术后3年或5年死亡的喉癌组织中PD4、Ki67阳性表达率均显著高于早期(Ⅰ～Ⅱ期)、无颈淋巴结转移及3年或5年存活病例(P﹤0.01或0.05);Ki67和P53蛋白在人喉癌组织中联合表达阳性率显著高于喉其他不同病变组织中联合表达阳性率(P﹤0.01)。结论Ki67和P53联合表达与人喉癌的发生、发展及预后有一定的相关性。
Objective To study the expression of Ki67 and P53 protein in laryngeal carcinoma and their relationship with the pathogenesis, development and prognosis. Methods Immunohistochemistry was used to study tissues of 141 cases with laryngeal carcinoma,18 cases with precarcinoma, 15 cases with vocal cord polyps and 8 normal tissues adjacent to laryngeal carcinoma. Results A high level of Ki67 and P53 expression was found in laryngeal carcinoma than in non-carcinoma laryngeal lesions and normal tissues （P 〈 0.01）. The expression rate of Ki67 and P53 protein were stronger in 3rd and 4th stage laryngeal carcinoma than those of the first and second stages. Laryngeal carcinoma with cervical metastasis had higher expression than those without cervical metastasis. The 3 years and 5 years survival rate of Ki67 and P53 positive cases was lower than that of Ki67 and P53 negative cases （P 〈 0.01 or 0.05）. The overall positive rate was higher in carcinoma than in non-carcinomal laryngeal lesions and normal tissues （P 〈 0.01）. Conclusions The high expression of Ki67 and P53 protein maybe closely related to the pathogenesis, development and prognosis of laryngeal carcinoma.
出处
《北京医学》
CAS
2007年第10期577-579,共3页
Beijing Medical Journal
基金
本课题为国家自然科学基金资助项目(编号30371610)
