摘要
目的:研究BAG-1和p53基因在喉鳞状细胞癌组织中的表达,探讨BAG-1在喉鳞状细胞癌发生、发展中的作用及与p53的关系。方法:运用免疫组织化学SP技术,检测40例喉鳞状细胞癌组织,27例喉癌旁正常组织及20例声带息肉组织中BAG-1与p53的表达。结果:BAG-1在喉癌中表达阳性率为72.5%,较喉癌旁组织(18.5%)、声带息肉组织(20.0%)高(P<0.05),其表达与预后呈负相关(P<0.05);p53在喉癌中表达阳性率为65.0%,而在喉癌旁组织和声带息肉组织中均无表达,与在喉癌组织中的表达相比差异有统计学意义(P<0.05);其表达与病理分化程度、颈淋巴结转移及预后呈负相关(P<0.05);在喉癌组织中BAG-1与p53表达之间未见显著性相关(P>0.05)。结论:BAG-1和p53基因的过度表达与喉鳞状细胞癌的发生、发展存在一定的关系。BAG-1可作为早期诊断喉癌的一种生物标记,尤其是细胞核BAG-1定位可作为一种不良预后的预测因子;p53表达与喉癌颈淋巴结转移有关,可单独作为喉癌的预后指标。
Objective： To study the expression of BAG-1 and p53 in laryngeal squamous cell carcinoma （LSCC）, and the relation between BAG-1 and p53. Method：BAG-1 and p53 protein were examined by using the method of immunohistochemical SP staining method in 40 cases of LSCC,27 cases of pap- a-cancerous tissues and 20 cases of polyp specimens from vocal cord tissues. Result.. The positive expression of BAG-1 in LSCC was significantly higher than that in para cancerous tissues and polyp specimens from vocal cord tissues （ P〈0.05）. The positive expressions of BAG-1 were related to the prognosis（ P〈0.05） ,and BAG-1 in LSCC with nucleus staining had worse prognosis than that with cytoplasm staining（ P〈0.05）. The positive expression of p53 in LSCC was significantly higher than that in para-cancerous tissues and polyp specimens from vocal cord tissues（ P〈0.05）. The positive expressions of p53 were related to the differentiation grades, cervical lymph node metastasis and prognosis（ P〈0.05）. There was no significance correlation between BAG-1 and p53 expression（ P〉0. 05）. Conclusion：Over expression of BAG-1 and p53 play an important role in the process of development of LSCC; BAG-1 may be a biological predictor of early period in LSCC,especially nuclear BAG-1 localization may be a prediction of unfavorable outcome. Expression of p53 is related with cervical lymph node metastasis, and that is a prognostic target.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第20期932-935,共4页
Journal of Clinical Otorhinolaryngology
基金
湖北省卫生厅资助项目(No:WJ01568)
关键词
喉肿瘤
癌
鳞状细胞
BAG-1
P53
基因表达
Laryngeal neoplasms~ Carcinoma, squamous cell~ Bcl-2-associated athanogene-1 p53~ Gene expression
