摘要
目的探讨槲皮素对巨噬细胞氧化应激反应与功能的调节作用。方法采用原代培养小鼠腹腔巨噬细胞和LPS诱导氧化应激,以20-80μmol·L^-1槲皮素处理48 h,测定细胞培养上清液抗氧化力、一氧化氮（NO）浓度、乳酸脱氢酶（LDH）活性、细胞活性氧含量（ROS）、MDA水平以及吞噬功能的变化。结果LPS对照组细胞培养上清液抗氧化力、NO浓度及LDH活性分别高于正常对照组20.6%、706.6%、27.6%;同时细胞ROS含量、NOS活性、MDA水平也分别高于正常对照组72.9%、331.3%、131.5%,细胞培养上清墨汁潴留也显著增加（A430由4.7±0.6增加到5.5±0.3,P〈0.01）。施加20-80μmol·L^-1的槲皮素后,与LPS对照组比较,上清抗氧化力升高31.0%-50.6%,上清NO浓度下降18.0%-87.0%,细胞ROS含量、NOS活性及MDA水平分别下降37.4%-72.1%、10.1%-25.4%、15.5%-74.8%。上清LDH活性在20-50μmol·L^-1皮素组下降21.9%-24.3%,在80μmol·L^-1皮素组则升高19.6%。细胞培养上清墨汁潴留减少,与正常对照组比较差异无显著性。结论一定浓度的槲皮素可增强小鼠巨噬细胞的抗氧化能力和吞噬能力,并降低氧化应激反应。
Objective To investigate the regulatory effects of quercetin on oxidative stress and function of mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were harvested and cultured, and were divided into normal contol, LPS（ to induce oxidative stress）, LPS ＋ 20 μmol·L^-1, 50 μmol·L^-1, and 80μmol·L^-1 of quercetin respectively. Antioxidative capacity（by FRAP method, and indicated by FeSO4 equivalent）, NO level, LDH activity in culture supematant, and cellular ROS level （by DCFH fluorescence probe method, indicated by relative fluorescence intensity RFI）, NOS actitivity, and phagocytic function （by ink phagocytosis method） were measured. Results The antioxidarive capacity, NO level, and LDH activity were 20.6% ,706.5%, 27.6% more respectively in LPS control group than in normal control group. The ROS content, NOS activity, MDA level were 72.9, 331.3 %, 131.5 % more respectively in LPS control group than in normal control group as well. The retention of ink in culture supernatant was also increased （A430：5.5 ± 0.3 vs 4.7 ± 0.6, P 〈 0.01 ）. When 20 - 80 μmol·L^-1 of quercetin were added to the culture medium, the antioxidative capacity increased 31.0% - 50.6% more, the content of NO, ROS, MDA, and NOS activity decreased 18.0% - 87.0%, 37.4% - 72.1%, 15.5% - 74.8%, and 10.1% - 25.4% more respectively than those in LPS control group. Moreover, LDH activity in 80 μmol·L^-1 of quereetin ＋ LPS group increased 19.6% more while that in 20 -50 μmol·L^-1 of quercetin ＋ LPS groups were decreased 21.9% -24.3% more than in LPS control group. The phagocytic function of macrophages after quercetin treatment was not significantly different from normal control. Conclusion Certain concentration of quercetin may enhance the antioxidative capacity and phagocytosis, and reduce LPS-induced oxidative stress in mouse peritoneal macrophages.
出处
《解放军预防医学杂志》
CAS
北大核心
2007年第3期160-163,共4页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
槲皮素
巨噬细胞
氧化应激
抗氧化
quercetin, macrophage, oxidative stress, antioxidation
