摘要
目的:研究中药五环三萜类有效成分熊果酸和齐墩果酸抗人肺癌细胞增殖、侵袭的作用,并探讨其抗侵袭作用的机理。方法:用熊果酸和齐墩果酸分别作用于克隆化高转移人肺癌细胞(PGCL3),测定细胞增殖抑制率、软琼脂集落形成率、对重建基底膜的侵袭能力、趋化运动能力、对层粘连蛋白的粘附能力以及组织蛋白酶B活性分泌等多项指标。结果:熊果酸和齐墩果酸均可降低PGCL3细胞增殖能力(P<0.001),IC50分别为44.73μmol/L和40.71μmol/L;30μmol/L、40μmol/L熊果酸和35μmol/L、45μmol/L齐墩果酸均能抑制PGCL3细胞的侵袭能力(P<0.001)且有剂量依赖性。抗侵袭作用机理的分析结果显示上述浓度的两种药物对细胞的运动、粘附能力及组织蛋白酶B分泌均有明显的抑制作用且均有统计学的显著意义,软琼脂集落形成率也显著降低。结论:熊果酸和齐墩果酸均有抗人肺癌细胞增殖和侵袭能力,其抗侵袭作用不是对侵袭的某一环节的阻断,而是对侵袭各个基本环节的全面抑制作用。
Objective :To investigate the effects of Ursolic acid (UA ) and oleanolic acid(OA) , both the active components of pent cyclic triterpene acid, on proliferation inhibition, anti - invasive activity in highly metastasis lung cancer cell line (PGCL3) ,and to make some analysis on their anti -invasion mechanism. Methods: We tested the cell proliferation inhibition rate, colony -formation rate in soft agar ,the invasive ability wit matrigel reconstituted basement membrane model, the chemo-tatic migration activity, the laminin adhesion ability and the secretion activity of cathepsin B after treatment of UA and OA in PGCL3 cell Iine respectively. Results : Both UA and OA can decrease the cell proliferation rate significantly, and the IC50 value is 44.73μmol/L and 40.71μmol/L respectively, and 30μmol/L and 40μmol/L UA, 35μmol/L and 45μmol/L OA can repress obviously the invasive ability of PGCL3 cell line in a dose - dependent manner. We made some analysis and found which made inhibitive effect to the migration ability, adhesion ability and the secretion of cathpsin B in a concentration - dependent manner, also the colony -formation rate in soft agar. Conclusions: Both two drugs can inhibit the proliferation ability and invasive ability, it showed that acted on cell not through any one point only of invasion but likely through all.
出处
《中医药学刊》
2004年第11期2065-2067,共3页
Study Journal of Traditional Chinese Medicine
基金
广州市科技攻关项目(98-Z-041-01)
关键词
熊果酸
齐墩果酸
人肺癌细胞
增殖
侵袭
Ursolic acid
Oleanolic acid
PGCL3
Proliferation
Invasion
