摘要
[目的]探讨肝癌微环境中Treg细胞的数量增多与HCC临床分期晚的可能原因。[方法]双重酶标免疫组织化学方法检测52例HCC组织中CD4+CD25+Treg细胞以及CD4+CD25-T(CD4+T)细胞分布,免疫组化EnVision法检测20例HCC组织中CD8+T细胞分布。[结果]正常肝脏组织中未发现Treg细胞,HCC组织中Treg细胞数量较癌旁组织明显增多(P=0.000),且肝癌组织中Treg细胞的数量与其浸润性CD4+T淋巴细胞的数量以及CD4+T/CD8+T比值呈显著负相关(r=-0.539,P=0.014;r=-0.545,P=0.000),而与浸润性CD8+T淋巴细胞的数量无明显相关性(r=-0.403,P=0.078)。[结论]Treg细胞在体内可能通过细胞接触的方式抑制CD4+T淋巴细胞的增殖来抑制肿瘤局部的免疫,去除或减少HCC微环境中浸润性Treg细胞数量可能提高肿瘤局部免疫治疗效果。
[Purpose ] To explore the causes of an increased prevalence of regulartory T（Treg） cells in microenvironment of hepatocellular carcinoma（HCC） which involved in TNM stage according to disease progression. [Methods] The CD4^＋CD25^＋ Treg and CD4^＋CD25^＋-T （CD4^＋T） cells in the slides tissues of 52 cases with HCC were marked by Double-immunohistochemical, and CD8＋T cells in the slides tissues of 20 cases with HCC by EnVision. [Results ] There was no Treg cell in normal liver tissues. The number of Treg cells in the tissue of HCC was significantly more than that in the tissue of peri-cancer （P=0.000）. The number of Treg cells in HCC tissue was negatively correlated to the ratio of CD4^＋T/CD8^＋T and CD4^＋T cells infiltration （r=-0.539, P=0.014 ;r=-0.545, P=0.000）, however, with no correlation to CDdT cells infiltration （r=-0.403,P=0.078）. [Conclusions ] Treg cells possibly suppress proliferation of CD4^＋T cells in a contactdependent manner so that tumor cells can escape immune surveillance and result in invasion and progression. Functional deletion of tumor-infiltrating Treg cells in mieroenvironment of HCC can enhance tumorspecific immunotherapy.
出处
《中国肿瘤》
CAS
2007年第6期461-463,共3页
China Cancer
