摘要
以不同提取方法制备的富硒灵芝提取物为试样,采用ESR技术比较了富硒灵芝不同提取物清除羟自由基(·OH)和超氧自由基(O-2·)的抗氧化活性及提取物之间抗氧化活性的相互作用,结果表明:富硒灵芝的水提物、醇提物和酶提物均具有清除·OH和O2-·的能力,呈明显的剂量-效应关系,清除率与提取物浓度之间可用对数曲线拟合。3种提取物对·OH的清除能力由强到弱依次为水提物﹥酶提物﹥醇提物;对O-2·的清除能力则依次为醇提物﹥水提物﹥酶提物。所有提取物对O-2·的清除活性均高于对·OH的清除活性。此外,3种提取物之间不存在清除O2-·的协同作用,但可以彼此增强其清除O2-·的活性。在清除·OH作用方面,醇提物与酶提物之间相互抑制,醇提物与水提物之间彼此增强,水提物与酶提物之间则几近相互协同。
Hydroxyl radical （.OH） and superoxide radical （O2^-·） scavenging activities of different extracts from Seenriched Ganoderma lucidum （Se-GL） and their interaction were compared by ESR. The results showed that water extracts, ethanol extracts and enzyme extracts from Se-GL all have the ability to attenuate .OH and O2^· in dose dependent manner. There was a logarithmic relationship between the concentrations of extracts and their radical scavenging ac- tivities. The activity of scavenging hydroxyl radical is in the order of water extracts 〉 enzyme extracts 〉 ethanol extracts while the activity of scavenging superoxide radical is in the order of ethanol extracts 〉 water extracts 〉 enzyme extracts. Additionally, water extracts, enzyme extracts and ethanol extracts could enhance each other in attenuating O^2-. However in scavenging ·OH, enzyme extracts and ethanol extracts refrain each other. Water extracts and ethanol extracts and water extracts and enzyme extracts enhance or synergistically enhance each other respectively.
出处
《中国食品学报》
EI
CAS
CSCD
2007年第2期11-16,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家博士后基金(2005037399)
浙江省博士后科研项目择优资助项目(2004-bsh-017)
关键词
富硒灵芝
提取物
清除自由基活性
Se-enriched Ganoderma lucidum Extracts Radical scavenging activities
