摘要
目的:观察丁苯酞(NBP)对脑缺血再灌注损伤后的神经保护作用及caspase-3表达的影响。方法:SD大鼠46只随机分成假手术组(n=6)、缺血再灌注组(n=10)、NBP大剂量组(80mg·kg-1,n=10)、NBP中剂量组(40mg·kg-1,n=10)和NBP小剂量组(20mg·kg-1,n=10),采用ZeaLonga法制作局灶性脑缺血再灌注大鼠模型,观察NBP对大鼠脑缺血再灌注后的神经功能症状、组织形态学改变、以及对caspase-3表达的影响。结果:与假手术组相比,缺血再灌注组大鼠出现严重的神经功能缺失症状,光镜下脑组织出现明显的梗死缺血灶,皮质和海马区caspase-3表达增强;与缺血再灌注组比较,NBP治疗组能显著改善大鼠神经功能缺失症状,减少脑组织的梗死缺血损伤,降低caspase-3的表达,其中以NBP大剂量组的神经保护作用最为显著(P<0.001)。结论:NBP对大鼠局灶性脑缺血再灌注损伤具有保护作用,其作用机制可能与抑制caspase-3表达相关。
Aim： To observe the effect of dl-3n-butylphthalide （NBP） on the expression of caspase-3 in the rat model of focal cerebral ischemia-reperfusion. Methods： Forty-six male SD rats were randomly divided into sham operation group （n=6）, ischemia-reperfusion （I-R） group（n=10）, NBP high-dose （80 mg-kg^-1） group （n=10）, NBP middle-dose （40 mg-kg^-1） group （n=10） and NBP low-dose （20 mg-kg^-1） group （n=10）. The rat model of focal brain ischemia-reperfusion was established with the suture-occluded method introduced by Zea Longa. The neurological deficit score, histological changes of brain and the expression of caspase-3 in cortex and hippocampus were detected. Results： There were significant neurological deficits, histological changes and elevated expression of caspase-3 in I-R group compared with sham operation group. The neurological deficit score, histological changes and the expression of caspase-3 in cortex and hippocampus were significantly decreased （P 〈 0.01） in NBP group compared with I-R group. Moreover, this neuroprotection was more significant in NBP high-dose group than in middle- or low-dosegroup （P 〈 0.01）. Conclusion： NBP can relieve the brain damage induced by focal I-R in rats, which may be correlated with the inhibition of expression of caspase-3.
出处
《中国临床神经科学》
2007年第1期19-23,共5页
Chinese Journal of Clinical Neurosciences
