摘要
目的研究丁苯酞对缺血性脑损伤产生作用的准确细胞类型。方法分别使用大鼠肾上腺髓质嗜铬细胞瘤细胞(Rat adrenal pheochromocytoma,PC12)和大鼠动脉内皮细胞(rat artery endothelial cells,SUVRAEC)构建氧气葡萄糖剥夺-再灌注损伤模型,通过噻唑蓝[3-(4,5-dimethylthiazole-2-y1)-2,5-biphenyl tetrazolium bromide,MTT]进行细胞活性检测,观察单纯损伤细胞与各剂量丁苯酞处理后的细胞活性。同时,采用1,1-二苯基-2-苦基偕腙肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法检测自由基清除活性。结果丁苯酞各剂量组对PC12和SUVRAEC的细胞活性均无明显影响,均不能抑制自由基离子。丁苯酞处理使经受氧气葡萄糖剥夺-再灌注损伤的SUVRAEC细胞活性增加约15%,约90%的细胞活性得到有效保护,并具有剂量依赖性;而对PC12的细胞活性则无明显作用。结论丁苯酞对PC12和SUVRAEC均无毒性作用,不具有直接清除自由基的能力;丁苯酞主要作用于血管内皮细胞,而对神经元的作用相对较弱。
Objective To investigate the potential cell types for neuroprotection of DL-3-n-butylphthallde against ischemic brain injury. Methods The Oxygen-Glucose Deprivation- Reperfusion models were created in rat adrenal pheochromocytoma （PC 12） cells and rat artery endothelial cells （SUVRAEC） and the effect of DL- 3-n-butylphthalide on stroke in different cell types was assessed using 3-（4,5-dimethylthiazole-2- yl）-2,5-biphenyl tetrazolium bromide （MTT） assay. The direct free-radical scavenging ability of DL-3-n-butylphthalide was evaluated using 1,1-diphenyl-2-picrylhydrazyl （DPPH） assay. Results Different concentrations of DL-3-n-butylphthalide （1/10/100μmol/L） did not have any negative effect on cell viability. DPPH assay did not reveal any direct free-radical scavenging ability of DL-3-n-butylphthalide. DL-3-n-butylphthalide concentration-dependently attenuated OGD-induced decrease in the cell viability in SUVRAEC but not neuron-like PC12 cells. DL-3-n- butylphthalide at an effective concentration could rescue 15% more cells than groups without it and approximate 90% SUVRAEC in total were alive. Conclusion DL-3-n-butylphthalide selectively protects SUVRAEC against ischemic injury and does not have a direct free radical scavenging property.
出处
《中国卒中杂志》
2010年第2期119-125,共7页
Chinese Journal of Stroke
关键词
丁苯酞
卒中
神经元
内皮细胞
DL-3-n-butylphthalide, Stroke, Neuron, Endothelial cells
