摘要
目的探讨神经节苷脂体外诱导骨髓基质干细胞(BMSC)向神经元样细胞分化的可能性。方法分离培养人BMSC,采用含神经节苷脂的无血清培养基诱导BMSC的分化,免疫细胞化学方法鉴定诱导分化后细胞表面神经细胞标志物神经元特异性烯醇化酶(NSE)和神经丝蛋白(NF)的表达。结果神经节苷脂诱导培养6h后,细胞表现为典型神经细胞样形态,神经元特异性标志物NSE和NF呈阳性表达。结论神经节苷脂可在体外诱导人BMSC分化为神经元样细胞。
Objective To investigate the possibility that bone marrow mesenchymal stem cells （BMSCs） differentiated into neuron-like cells induced by ganglioside in vitro. Methods BMSCs were separated, cultured and differentiate into neuron-like ceils induced by ganglioside. Neuro-specific enolase （NSE） and neurofilament （NF） on the surface of differentiated and induced BMSCs were detected by immunocytochemistry. Results BMSCs were induced to differentiate into the cells with a typical neuronal morphology. The induced neuron-like cells expressed NSE and NF. Conclusion BMSCs can be differentiated into neuron-like cells by induction of ganglioside in vitro.
出处
《中国康复理论与实践》
CSCD
2006年第12期1054-1055,F0003,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
骨髓基质干细胞
神经节苷脂
诱导分化
神经元样细胞
bone marrow mesenchymal stem cells （BMSCs）
ganglioside
induced differentiation
neuron-like cells
