摘要
目的:评价经腺病毒介导的血管内皮细胞生长因子(VEGF)基因转染后的大鼠骨髓基质干细胞(BMSCs)诱导成内皮细胞的可行性。方法:从大鼠骨髓中分离培养获取BMSCs,分别经Adv-VEGF、Adv-GFP转染,Western印迹分析检测VEGF蛋白的表达情况,细胞生长曲线测定BMSCs增殖特性,同时进行免疫表型鉴定,并与未转染细胞组比较。结果:AdvVEGF转染组BMSCs可分泌VEGF蛋白,而其余两组不分泌VEGF蛋白。转染VEGF基因后,BMSCs生长速度变快,向内皮细胞分化。与Adv-VEGF转染组BMSCs相比,Adv-GFP转染细胞组、未转染细胞组的BMSCs生长速度较慢,未有向内皮细胞分化的证掘。结论:腺病毒介导的VEGF基因转染可诱导BMSCs分化成内皮细胞。
Objective: To investigate the feasibility of using vascular endothelial cell growth factor (VEGF) gene to in duce hone marrow stromal stem cells(BMSCs) into endothelial cells in vitro. Methods :BMSCs were isolated from mouse bone marrow and cultured in vitro. They were divided into 3 groups : Adv- VEGF transfected group, Adv-GFP transfected group and un-transfeeted group. Western blot analysis .cell growth curves determination, and immunohistochemistry were performed. Results: After induced with Adv-VEGF, BMSCs grew faster and differentiated into endothelial cells. Compared with the Adv-VKGF transfected group. BMSCs in other groups grew slowly and didnot differentiate into endothelial cells. In Adv-VKGF transfected group. Western blot analysis showed that these cells indeed produced the VEGF protein,but Adv-GFP transfected group and un transfected group didnot. Conclusion : Adenovirus mediated VEGF gene transfection can induce endothelial cell from BMSCs.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第12期1280-1283,共4页
Academic Journal of Second Military Medical University
基金
全军医药科研"十五"规划重点课题(01Z060)
关键词
血管内皮细胞生长因子
基因转染
大鼠
骨髓基质干细胞
细胞分化
内皮细胞
组织工程
心脏瓣膜
bone marrow stromal stem cells
vascular endothelial cell growth factor
transfection
cell differentiation
endothelial cells
tissue engineering
heart valve
