摘要
目的测定妊高征患者胎盘滋养细胞线粒体ATP酶活性及氧自由基的变化,探讨其在妊高征发病机制中的作用。方法选取妊高征患者(53例)及正常妊娠妇女(32例)胎盘,匀浆后提取线粒体,分别测定线粒体Ca2+-Mg2+-ATP酶、SOD的活性及MDA含量变化。结果重度妊高征患者胎盘滋养细胞线粒体Ca2+-Mg2+-ATP酶、SOD活性与正常孕足月胎盘相比明显降低,而MDA含量却明显升高(P<0.05);Ca2+-Mg2+-ATP酶活性与MDA含量呈负相关,而与SOD呈正相关。结论重度妊高征患者胎盘滋养细胞线粒体ATP酶活性显著降低,自由基增多,提示自由基抑制ATP酶活性,促进妊高征病情进展。
[Objective] To investigate the role in pathogenesis of pregnancy induced hypertension （PIH）. [Methods] 53 women with PIH （25 mild or moderate, 28 severe PIH） and 32 women with normal pregnancy were recruited as study group and control, respectively. The mitochondria of placenta cytotrophoblasts were estracted and the activity of the enzyme and concentration of malondialdehyde （MDA） were measured. [Results] The activity of Ca^2＋- Mg^2＋- ATPase and SOD of mitochondria of placenta cytotrophoblasts in severe PIH were lower than control, while the activity of MDA was higher than control （P 〈0.05）. Positive correlation between Ca^2＋- Mg^3＋-ATPase and SOD, but negative correlation between Na^＋-K^＋-ATPase, Ca^2＋- Mg^2＋-ATPase and MDA were found both in study group and control group. [Conclusion] Decrease of the activity of ATPase but increase of oxygen-derived free radidicals of placenta cytotrophoblasts mitochondrion in patients With pregnancy induced hypertension suggested that free radical suppressed ATPase and facilitate PIH.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第18期2782-2784,共3页
China Journal of Modern Medicine
