摘要
目的建立辣椒风湿膏质量标准。方法采用SP-2000A气相色谱仪制定薄荷脑、冰片的鉴别方法。采用KromasilC18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.17%磷酸水溶液,采用梯度洗脱;流速:1.0ml/min;检测波长:209nm,对主药辣椒的活性成分辣椒素进行了含量测定。结果薄荷脑、冰片的保留时间与对照品主成分保留时间一致。辣椒素对照品在0.03952～0.3952μg范围内呈良好的线性关系(r=0.9995),平均回收率为96.61%,RSD为0.30%。结论采用方法简便、专属性强、重复性好,可用于该制剂的质量控制。
Objective To develop the quality standard for Lajiao Fengshi Plaster. Methods Identification for menthol and bomeol were established by gas chromatography SP-2000A. The content of capsaicin, an activity component from capsicum which is the main ingredient of Lajiao Fengshi Piaster, was determined by Kromasil C^s chromatogram columniations （250 mm × 4.6 mm, 5 μm） mobile phase of aqueous acetonitrile-0.17%phosphoric with gradient elution method, velocity of flow： 1.0 ml/min, and detection wavelength： 209 nm. Results The retention time of menthol and borneol is consistent with that of the main ingredient of the reference substance. The good liner range of capsaicin reference substance on calibration curve was 0.03952-0.3952 μg （r=0.9995）, and the average recovery was 96.61%, RSD=0.30%. Conclusion The method is sensitive, specific, and repeatable. It could be used in quality control for Lajiao Fengshi Plaster.
出处
《国际中医中药杂志》
2006年第4期214-216,共3页
International Journal of Traditional Chinese Medicine
