摘要
目的将皮瓣延迟与采用血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)对皮瓣成活的影响进行对比研究。方法3月龄SD大鼠30只,随机分为生理盐水组、皮瓣延迟组及VEGF组,每组10只。应用背部超长、宽比随意皮瓣模型。皮瓣延迟组采用双蒂皮瓣延迟,延迟时间为7d,之后断头端蒂,形成蒂部位于尾端的单蒂皮瓣;VEGF组形成单蒂皮瓣,于皮瓣中、远段均匀分为4点,局部皮下注射含400ngVEGF溶液100μl;生理盐水组于局部皮下注射生理盐水100μl,余同VEGF组。单蒂皮瓣完全形成后5d,计算皮瓣成活率,切取皮瓣组织,进行微血管密度分析、微血管直径测量和微血管断面面积测量。结果VEGF组皮瓣成活率与皮瓣延迟组接近,差异无统计学意义(P>0.05)。皮瓣延迟组内部微血管平均直径明显大于VEGF组和生理盐水组,VEGF组皮瓣内部微血管密度明显大于生理盐水组和皮瓣延迟组,差异均有统计学意义(P<0.05)。皮瓣延迟组和VEGF组相比,其微血管断面面积接近,差异无统计学意义(P>0.05)。结论皮瓣延迟后皮瓣内部主要表现为微血管扩张,应用VEGF后,皮瓣内部主要表现为微血管增生。二者均能有效增加皮瓣内部微血管断面面积,提高皮瓣成活率,但其作用途径不同。
Objective To compare the effects of flap delay and vascular endothelial growth factor （VEGF） on the viability of the rat dorsal flap. Methods Thirty rats were divided into 3 groups ： saline group, flap delay group and VEGF group. The rats in flap delay group underwent flap delay by keeping bipedicle untouched, and the cranial pedicle was cut 7 days later. The rats in VEGF group were given VEGF solution locally when the flaps were elevated in the operation. The rats in saline group were given saline solution in the same way. Five days after the single pedicle flaps were performed, the flap survival rate was measured. The flap tissues were collected to measure and analyze the microvascular density, diameter and sectional area by immunochemical method. Results The flap survival rate of flap delay group was similar to that of VEGF group and there is no statistically significant difference （P〉 0.05）. The vascular diameter of flap delay group was much larger than that of saline group and VEGF group, showing statistically significant difference （P〈0.05）. The vascular density of VEGF group was much higher than that of saline group and flap delay group, showing statistically significant difference （P〈0.05）. The vascular sectional area of flap delay group was similar to that of VEGF group （P〉 0. 05）. Conclusion The change in the flap after flap delay is manifested as obvious dilatation of microvessels, while the change in the flap after the injection of VEGF is manifested as obvious vascular proliferation. Both flap delay and VEGF can increase the vascular sectional area and the viability of the flap, but the mechanism is different.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第5期530-533,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
湛江市科技攻关资助项目(2002-17)~~
关键词
皮瓣延迟
血管内皮细胞生长因子
皮瓣成活
比较研究
Flap delay Vascular endothelial cell growth factor Flap survival Comparison study
