摘要
作者通过抽取１５例临床应用异种（猪）骨基质明胶ｐＢＭＧ病人的抗血清，在ｐＢＭＧ石蜡切片上进行免疫组化酶染色，来了解和定位ｐＢＭＧ的抗原性，从而推测病人体内是否产生了特异性抗体。实验结果表明，ｐＢＭＧ内没有棕红色阳性染色区，其染色情况与阴性对照组完全一致，这说明ｐＢＭＧ经过特殊的制备后，已没有可以检测出的抗原性，而病人体内也没有发现相应的特异性抗体产生。
Accordingtoreportsintheliterature,porcinebonematrixgelatinpBMGhadbeenusedclinicalyforthetreatmentofbonedefectsandnonunionoffractures.However,afterspecialtreatmentofporcinebonematrixgelatinpBMG,istherestilanyantigenicitypresent?Furthermore,isthereanyproductionofantibodyinthepatient'sbodyreceivingtheagent?Thisstudywascarriedouttoanswertheabovequestions.Theserumof15patientswithimplantationofporcinebonematrixgelatinpBMGwasstudiedbyimmuno-histochemicalmethodsontheslidesofpBMG.Thesespecimenswereobtainedduringreoperationbecauseofrefracture.SpecimensofpBMGnotimplantedintothehumanbodyweremadeintoslidesof5μmthicknessandusedascontrols.Theresultsshowedthat,therewasnotanypos-itivestainedreddishzonesintheslidesofpBMGeitherintheimplantedspecimensorinthecontrols.Therewasnodiferencebetweentheexperimentalandthecontrolgroups.ItwassuggestedthatafterspecialtreatmentnoantigenexistedinpBMGandnoantibodywasproducedinpBMGreceivingpa-tients.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第6期392-394,共3页
Chinese Journal of Orthopaedics
