摘要
目的 :探讨胃癌微卫星不稳定性 (MSI)与胃腺癌中血管内皮生长因子 (VEGF)之间的关系。 方法 :应用单链构象多态性 聚合酶链反应 (singlestrandconstructionpolymorphism PCR ,SSCP PCR)技术检测 30例胃腺癌患者 5个位点的微卫星不稳定性 ,同时应用免疫组化方法检测肿瘤VEGF的表达。 结果 :MSI阳性率为 4 3.4 % (13/ 30 ) ;VEGF阳性率为 6 0 % (18/ 30 )。高微卫星不稳定性 (MSI H)胃癌VEGF的表达显著减少。 结论 :MSI H胃癌与微卫星稳定性 (MSS)胃癌可能存在两种不同的新生血管形成的途径。MSI H肿瘤较低的VEGF表达或许可解释为何MSI H胃癌有较低的侵袭性。
Objective:To investigate the correlation between the microsatellite instability (MSI) and the expression of vascular endothelial growth factor (VEGF) in gastric adenocarcinoma. Methods:Single strand construction polymorphism PCR (SSCP-PCR) method was used to detect MSI of thirty patients with gastric adenocarcinoma and samples were taken from five foci of the lesion. Expressions of VEGF were examined by immunohistochemistry. Results:MSI was 43.4%(13/30) in our study .Positive signs of VEGF Immunostaining were detected in 18 patients (60.0%).VEGF was decreased in MSI-H gastric adenocarcinoma. Conclusion:MSI-H and MSS gastric adenocarcinoma may follow different pathway to angiogenesis.The low frequency of VEGF expression among MSI-H cancer may be one of the reasons for why these cancers are less aggressive.
出处
《医学研究生学报》
CAS
2005年第1期37-40,共4页
Journal of Medical Postgraduates
关键词
胃癌
微卫星不稳定性
血管内皮生长因子
Gastric cancer
Microsatellite instability
Vascular endothelial growth factor
