摘要
目的 观察乳化异氟醚单次腹腔注射对大鼠的麻醉作用及测定乳化异氟醚半数有效剂量(ED50)和半数致死剂量(LD50)。方法 130只SD大鼠随机分为13组,ED50组(内含6个亚组)、LD50组(内含6个亚组)及对照组,每亚组及对照组各10只,雌雄各半。其中ED50组为单次腹腔内注射不同浓度2.30％-6.00％乳化异氟醚1.5ml/100g,LD50组为单次腹腔内注射不同浓度4.09％-10.64％乳化异氟醚2ml/100g。对照组为单次腹腔注射纯30％脂肪乳2ml/100g。观察大鼠翻正反射、毒性反应和死亡。结果 ED50组和LD50组乳化异氟醚用量分别相当于液态异氟醚的(0.57±0.07)ml/kg和(1.26±0.10)ml/kg,95％可信区间分别为0.51-0.64和1.10-1.45ml/kg,ED50组麻醉起效时间为(2.6±1.0)min,作用时间为(28±11)min;治疗指数(TI)为2.24。结论 (1)单次腹腔注射乳化异氟醚在大鼠可产生有效的可逆的全身麻醉作用;(2)乳化异氟醚腹腔注射为短时间(20-30min)动物实验提供一新的麻醉给药途径;(3)单次腹腔注射乳化异氟醚ED50、LD50测定为该麻醉方法的有效性和安全性提供实验依据。
Objective To investigate the anesthetic action of intraperitoneal (i.p.) emulsified isoflurane and determine ED50 and LD50 in rats. Methods One hundred and thirty SD rats aged 6-8 weeks veighing 120-150 g were randomly divided into three groups: (A) control group ( n = 10) ; (B) ED50 group ( n = 60) ; (C) LDSO group (n = 60) . Group B and C were further divided into 6 subgroups with 10 animals (5 male, 5 female) in each subgroup. In control group a single bolus of 30% emulsified fat 2ml/100g body weight was administered i. p. In ED50 group a single bolus of 2.30% -6.0% vol/vol emulaified isoflurane 1.5 ml/100 g was administered i. p. and righting reflex was recorded. In LD50 group a single bolus of 4.09% - 10.64% vol/vol emulsified isoflurane 2 ml·100 g-1 was given i.p. and toxic response and lethal dose were recorded. The concentration ratio of the two neighbouring subgroups was 1:0.825. Results The ED50 of emulsified isoflurane was (0.57±0.07) ml.kg-1 and 95% confidence limit (CL) was (0.51-0.64)ml·kg-1 . The average onset time of action was (2.64 ±0.99) min and the average duration of action was (28 ± 11) min. The LD50 of emulsified isoflurane was (1. 26 ±0.10) ml·kg-1 and 95% confidence limit was 1.10-1.45 ml·kg-1 . The therapeutic index (LD50/ED50)was 2. 24. Conclusion Intraperitoneal emulsified isoflurane can provide effective general anesthesia and can be used for animal experiments which need anesthesia of short duration.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2004年第1期51-53,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金资助(30271259)
