摘要
钙调素(CaM)是细胞内Ca^(2+)的主要受体,在细胞增殖、分化、凋亡、迁移等过程中都发挥着重要的调控作用。采用GFP标记技术,我们观察了GFP-CaM在胞质分裂期HeLa细胞中的动态分布,发现在胞质分裂后期,GFP-CaM与中体紧密相连。抑制CaM的活性会阻止中体的解聚。进一步观察发现,CaM与γ-微管蛋白共分布在中体两侧,抑制CaM活性也会引起中体γ-微管蛋白解离的延迟。本实验结果说明分布在中体上的CaM很可能通过影响中体微管的稳定,参与调控胞质分裂的完成。
Calmodulin (CaM) is a major cytoplasmic Ca2+ receptor and performs a multiplicity of functions in the cell. By using GFP-CaM fusion protein, we have studied the detailed dynamic redistribution of CaM during cytokinesis in HeLa cells. CaM associates with midbody in late cytokinesis phase. When the cells were treated with Ca2+ /CaM inhibitor W7,the dissolving of the midbody was delayed. Moreover, we have found that γ-tubulin colocalized with CaM at the midbody during cytokinesis. W7 treatment could affect the dissociation of γ-tubulin from midbody. These results suggest that CaM may involve in the regulation of midbody microtubules disassembly and may thus affect the completion of cytokinesis.
出处
《实验生物学报》
SCIE
CAS
CSCD
北大核心
2003年第5期335-341,共7页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(30170453)
教育部优秀青年教师资助计划项目(教人司[2001]39号)资助
