摘要
构建增强型绿色荧光蛋白(enhancedgreenfluorescentprotein,EGFP)基因的真核表达载体pCDNA3.1(+) EGFP,转染至培养的Hela细胞中成功表达,并发出绿色荧光,证明EGFP一种良好的报告基因和筛选标记.
The eukaryotic expression vector pCDNA3.1(+)-EGFP was constructed for determination of expression of enhanced green fluorescent protein gene in Hela cell. It was demonstrated that EGFP gene was successfully expressed in Hela cells, implying that EGFP was a good reporter and selection marker molecule in mammalian cells.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第2期386-388,共3页
Journal of Sichuan University(Natural Science Edition)
