摘要
目的 :探讨束缚应激对大鼠心肌细胞凋亡的影响及中药颐心宁抗应激性心肌损伤的作用。方法 :采用琼脂糖凝胶电泳和TUNEL检测心肌细胞凋亡。结果 :①束缚 1、2、4周时 ,应激组DNAladder心肌细胞凋亡结果阴性 ,原位细胞凋亡明显增多 ,统计学有非常显著差异 (P <0 .0 1) ;②颐心宁干预组DNAladder结果阴性 ,而原位细胞凋亡检测结果则较单纯应激大鼠显著减轻 (P <0 .0 5 ) ;③蒸馏水干预组与单纯应激大鼠比较则无统计学意义 (P >0 .0 5 )。结论 :束缚应激可诱导大鼠心肌细胞发生凋亡 ,中药颐心宁可抑制心肌细胞凋亡 ,具有抗应激性心肌损伤的作用。
Aim:To discuss the effects of restrained stress on cardiac myocyte apoptosis of rats in the whole body,and the effect of anti-stress-induced cardiac myocyte injury treated by Chinese herbs Yixinning.Methods: Agarose gel electrophoresis and TUNEL are used to detect cardiac myocyte apoptosis. Results: ①When restrained stress 1,2,4 week, the DNA ladder of stress groups was negative,while in situ apoptosis of stress groups increased apparently compared with control group(P<0.01).②The DNA ladder of Yixinning groups was negative too, while in situ apoptosis of Yixinning groups decreased compared with stress group(P<0.05).③Whereas in distilled water group, the above indices had no statistical significance compared with strees model group(P>0.05) .Conclusion: The restrained stress can induce cardiac myocyte apoptosis in rats.Chinese herbs 'Yixinning' can inhibit cardiac myocyte apoptosis,and have functions of anti-stress injury.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2003年第2期117-120,共4页
Chinese Journal of Applied Physiology
基金
全军医药卫生科研基金资助项目 ( 0 1L0 2 8- 4 )
