摘要
以白菜型油菜Hauarad(70 8)、茅山 3(714 )及油白 (715 )和甘蓝京丰 1号 (6 0 12 )为供体材料 ,对白菜型油菜和甘蓝杂交种进行胚胎挽救研究。对于杂交组合 70 8× 6 0 12和 714× 6 0 12 ,取授粉第 9天的子房培养获得的种子最多 ,结籽率分别为 17.5 %和 95 .0 %。而杂交 715× 6 0 12 ,以取授粉第 12天的子房进行培养得到的种子最多 ,结籽率为 5 0 .0 %。不同杂交组合所获得的种子数存在明显差异 ,以杂交组合 714× 6 0 12为最好 ,结籽率达 4 3.0 %。不同培养基上进行子房培养表明 ,取 70 8× 6 0 12的杂交子房 ,采用培养基C效果最好 ,结籽率达 4 4 .0 % ;对于 714× 6 0 12的杂交子房而言 ,A、B、C、D 4种类型培养基都有一定的效果 ,其中培养基A和B最好 ,结籽率分别达到了 6 4 .0 %和72 .0 %。而B培养基也对 715× 6 0 12的杂交子房培养效果最好 ,结籽率达 6 0 .0 %。所获得的种子在分化 (萌发 )培养基上培养表明 ,来自于杂交 715× 6 0 12的种子萌发效果最好 ,种子萌发率达 6 6 .7% ,并在生根培养基上得到了再生植株。对再生植株进行初步细胞学鉴定结果表明 ,其单倍体的染色体数均为 19。
Using three varieties of B. campestris, Hauarad (708), Maoshan-3 (714) and Youbai (715) as the maternal plants and one variety of B. oleracea Jingfeng1 (6012) as paternal plants, crosses were made to produce interspecific hybrids through ovary culture techniques. The ovaries from the cross between B. campestris × B. oleracea (708×6012 and 714×6012) were cultured and ovary culture was more effective in terms of seeds obtained when ovaries were in vitro culture at 9 days after pollination (DAP). While for the cross of 715×6012 it was better when ovaries in vitro culture at 12 DAP. Among three cross combinations, the cross of 714×6012 showed best response and 43 seeds per ovary were obtained. Of the media studied, the ovaries from the cross of 708× 6012 were cultured on MS media supplemented with 3.0 mg·L -1 6-BA+0.1 mg·L -1 NAA+0.5% activated carbon showed better response, and its rate of seeds per ovary reached 44.0%. While the ovaries from the other two crosses (714×6012 and 715×6012) showed best response with cultured on B5 media supplemented with 3.0 mg·L -1 6-BA+0.2 mg·L -1 NAA+0.5% carbon, and the rates of seeds per ovary reached 72.0% and 60.0%, respectively. All seeds obtained from the three cross combinations were cultured on the MS media supplemented with 1.0 mg·L -1 6-BA+0.05 mg·L -1 NAA, and the seeds from the cross of 715×6012 showed best germination response and the percentage of germinations reached 66.7%. The regenerated plantlets were obtained from these seedlings after culture on the MS media supplemented with 0.05 mg·L -1 NAA. Cytological study showed that these regenerated plants were all true hybrids of B. campestris × B. oleracea.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第11期1409-1413,共5页
Scientia Agricultura Sinica
基金
浙江省重点科技资助项目 (0 2 110 2 10 9)
中瑞政府间合作资助项目 (C0 8)
