摘要
目的 比较四种梅毒血清学实验诊断方法的准确性及在临床的应用价值。 方法 应用甲苯胺红不加热血清试验 (TRUST) ,梅毒密螺旋体血凝试验 (TPHA) ,梅毒酶联免疫吸附试验 (TP～ELISA)及梅毒抗体蛋白印记法(Western -blot)检测 2 5 0例患者血清。 结果 TRUST方法特异性及灵敏度较低 ;Western -blot特异性为 10 0 % ,但敏感性较差 ;TPHA与ELISA两种方法在检测梅毒抗体时灵敏度与特异性都比较好 ,而且相当接近。 结论 ELISA法因其准确性及方法学的优势 。
Objective The sensitivity and specificity of four kinds of serological approaches detecting treponema pallidum antibody were compared and the corresponding clinical application values were analyzed in this study Methods Four approaches i e TRUST, TPHA, TP～ELISA and Western-blot, were used to detect the serum specimens from 250 patients suffering from syphilis Results Among the four approaches, Western-blot was found to be the most efficient and the least sensitive one with the efficiency of 100%; Both TPHA and TP～ELISA were more sensitive and efficient than TRUST, moreover, there was no significant difference between TPHA and TP～ELISA Conclusion It is suggested that the approach of TP～ELISA should be employed first for detecting treponema pallidum antibody at present
出处
《中国热带医学》
CAS
2003年第6期825-826,共2页
China Tropical Medicine
