摘要
为了分析SARS(SevereAcuteRespiratorySyndrome)冠状病毒的密码子偏爱性(codonpreference),为SARS冠状病毒基因表达中宿主系统的选择提供参考.运用EMBOSS(TheEuropeanMolecularBiologyOpenSoftwareSuite)的CHIPS(CodonHeterozygosityinaProtein codingSequence)和CUSP(Createacodonusagetable)程序对SARS冠状病毒的6个编码蛋白的基因进行分析,并将这6个编码序列拼接在一起进行全基因组的密码子偏爱性分析.分析结果与大肠杆菌、酵母及人的密码子偏爱性进行比较.结果显示SARS冠状病毒的CHIPS分析Nc(effectivenumberofcodons)值为53.338,S、E、M、N蛋白、3CL水解酶、RNA聚合酶的Nc值分别为45.733,61.000,59.040,46.618,46.924,51.902.编码SARS冠状病毒A,P,R,S,T,L等氨基酸的不同密码子使用频率有较大差异.大肠杆菌有25个、酵母有12个、人有20个密码子与SARS冠状病毒密码子使用偏爱性差异较大.因此可以得出结论:编码SARS冠状病毒氨基酸的密码子出现的频率较均一.SARS冠状病毒的密码子偏爱性与真核生物较接近,与原核生物相差较远,其基因表达选择在酵母等真核系统可能更为合适.
Inorder to analyze SARS coronavirus' codon preference and search for a preferential selecting host systems for SARS coronavirus' gene expression. 6 coding sequences and the whole genome of SARS coronavirus were analyzed by CHIPS and CUSP programs of EMBOSS. The results were compared with E.coli, yeast, and human's codon usage. Results showed the Nc value of SARS coronavirus' genome and S, E, M, N, 3CLprotein, RNA polymerase are 53.338, 45.733,61.000, 59.040, 46.618, 46.924 and 51.902 respectively. Alternative codons for A, P, R, S, T, L amino acid in SARS coronavirus have distinctly different frequency. There are 25, 12, 20 codons' frequency of SARS coronavirus evidently disagreeing with E.coli, yeast, or human respectively. It could be concluded that Nc value demonstrated that the frequency of SARS coronavirus' codon was comparatively uniform. Codon preference of SARS coronavirus was close to that of the eukaryote and deviated from prokaryote. Yeast system should be more suitable for SARS coronavirus' gene expression.
出处
《生命科学研究》
CAS
CSCD
2003年第3期219-223,共5页
Life Science Research
基金
全军十五重大攻关项目资助
