摘要
运用EMBOSS(TheEuropeanMolecularBiologyOpenSoftwareSuite)的CHIPS(Condon HeterozygosityinaProteinCodingSequence)和CUSP(Createacondonusagetable)程序对WSSV3个编码蛋白的基因密码子偏爱性分析,分析结果与大肠杆菌、酵母及人的密码子偏爱性进行比较。结果显示,WSSV vp37、WSSV vp39及WSSV vp19的Nc(Effectivenumberofcondons)值为49.897、48.640、46.868。3种蛋白的编码密码子使用频率有较大的差异,其在大肠杆菌、酵母及人的密码子偏爱性也有不同,分析结果表明,WSSV vp37和WSSV vp39的密码子与原核生物及人的相差较大,其表达系统选择在酵母较为合适,而WSSV vp19的密码子与原核和真核生物酵母相差较大。
Three coding sequences of WSSV were analyzed by CHIPS and CUSPS programs of EMBOSS for identifying condon preference and selecting preferential host systems for gene expression. The results were compared with Escherichia coli, yeast and human＇s condon usage. Results showed that the Nc values of WSSV-vp37,WSSV-vp39 and WSSV-vp19 were 49. 897, 48. 640 and 46. 868, respectively. There have been distinctly frequency differences between WSSV-vp37, WSSV-vp39, and WSSV-vp19. Condon preferences were different from those of E. coli, yeast and human. Condon preferences of WSSV-vp37 and WSSV-vp39 were close to that of yeast system and deviated from E. coli and Arabidopsis thaliana like homo sapiens. Yeast system should be selected for WSSV-vp37 and WSSV-vp39 gene expression. But condon preference of WSSV-vp19 was obviously different from those of eukaryote and prokaryote.
出处
《海洋水产研究》
CSCD
北大核心
2005年第4期1-7,共7页
Marine Fisheries Research
基金
国家重点基础研究项目(G1999012002)
国家自然科学基金(30271020)
中国水产科学研究院项目(200341)资助
