摘要
目的 :应用体外受精 -胚胎移植 (IVF ET)技术治疗不孕症 ,探讨胚胎培养系统和不孕妇女年龄对治疗效果的影响。方法 :2 0 8对不育夫妇 ,女方采用控制性超排卵 ,阴道B超引导穿刺取卵 ,丈夫精液采用梯度离心法分离精子 ,体外受精 ,胚胎移植 ;比较不同培养系统和年龄对治疗效果的影响。结果 :2 2 0个治疗周期共获卵 2 0 97个 ,周期获卵数为 9.5 3± 5 .61个 ,受精率 72 .2 1% ,卵裂率 94.40 % ,优良胚胎率为 67.43 % ,96周期临床妊娠 ,临床妊娠率为 43 .64 % ,双胎率为 3 6.46% ,流产率为 13 .5 4%。结论 :IVF -ET技术是治疗不孕症的有效方法。通过改进培养系统 ,能够改善胚胎质量 ,提高IVF治疗的成功率 ;妇女年龄超过 3 5岁IVF治疗的成功率明显降低。
Objective To apply the technique of in vitro fertilization and embryo transfer (IVF-ET) to infertility treatment and discuss the effect of embryo culture system and female age on treatment outcomes. Methods 208 infertile couples were accepted. Oocytes were collected from females with controlled ovarian stimulation. Sperms were collected from husbands by density gradient centrifugation. After in vitro fertilization, embryos were transferred into uteruses. The effect of embryo culture system and female age on treatment outcomes were discussed. Results 2097 oocytes were collected in 220 cycles, the oocyte number per cycle was 9.53±5.61. The fertilization rate was 72.21%, the cleavage rate 94.4%, the fine embryo rate 67.43%. Clinical pregnancy was achieved in 96 cycles. The pregnancy rate was 43.64%. Among which, the twin embryos rate was 36.46%, the abortion rate 13.54%. Conclusions The technique of IVF-ET is an efficient method for infertility treatment. The pregnancy rate can be increased by improving the embryo culture system and embryo quality. The pregnancy rate decreases in female over 35.
出处
《中原医刊》
2003年第17期1-3,共3页
Central Plains Medical Journal
基金
广东省中山市科委基金资助 (A0 4 8)
