摘要
以 9个菊花品种为试材 ,利用 6种分化培养基和 3种生根培养基 ,以叶片、茎段为外植体 ,研究不同基因型、激素组合以及附加不同浓度的AgNO3 对高效、快速、经济的再生体系建立的影响。结果表明 ,最适分化培养基分别为 :‘日本黄’ :6 BA0 .5mg/L +NAA0 .1mg/L或 6 BA2 .0mg/L +NAA0 .5mg/L ;‘9718’、‘RH3’、‘寒紫’、‘寒黄’ :6 BA2 .0mg/L +NAA0 .5mg/L ;‘日本白’ :6 BA0 .5mg/L +NAA0 .1mg/L ;‘寒红’、‘杭白菊’ :6 BA1.0mg/L +NAA0 .1mg/L ;‘五九菊’ :6 BA2 .0mg/L +NAA0 .1mg/L。适宜生根培养基为 1/2MS +0 .1mg/L～ 0 .2mg/LNAA、或 0 .2mg/L～ 0 .4mg/LIAA、或 0 .4mg/L～ 0 .6mg/LIBA。AgNO3 对不定芽的分化有抑制作用。
This paper deals with efficient regeneration system from leaf pieces and internodal segments of 9 chrysanthemum cultivars with six differentiation media and three rooting media.The effect of genotype,combinations of plant growth regulators, concentrations of silver nitrate on differentiation was investigated .The results showed that optimized differentiation media were:'Japanese Yellow':6 BA 0.5 mg/L+NAA 0.1 mg/L or 6 BA 2.0 mg/L+NAA 0.5 mg/L;'9718'?'RH3'?'Winter Purple'?'Winter Yellow':6 BA 2.0 mg/L+NAA 0.5 mg/L;'Japanese White':6 BA 0.5 mg/L+NAA 0.1 mg/L;'Winter Red'?'Hang Bai Ju':6 BA 1.0 mg/L+NAA 0.1 mg/L;'Wu Jiu Ju':6 BA 2.0 mg/L+NAA 0.1 mg/L. Optimized rooting media were: 1/2MS+0.1～0.2 mg/L NAA?or 0.2～0.4 mg/L IAA?or 0.4～0.6 mg/L IBA?Silver nitrate restrained shoot regeneration.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2003年第2期162-166,共5页
Journal of Huazhong Agricultural University
基金
武汉市科学技术局技术计划项目 ( 2 0 0 2 5 0 0 7170 7)
关键词
菊花
品种
再生体系
叶片
茎段
遗传转化
离体培养
培养基
chrysanthemum, leaf piece, internodal segment, plant regeneration, silver nitrate
