摘要
目的 研究胰腺癌组织及细胞中Bax/Bcl-2蛋白和FHIT mRNA的表达,探讨FHIT与凋亡相关因子在胰腺癌中的关系。方法 应用ABC免疫组织化学方法检测胰腺癌细胞和组织标本中Bax与Be1-2蛋白,RT-PCR检测其中FHIT mRNA的表达。结果 38例胰腺癌中Bax蛋白阳性17例(45％),Bcl-2蛋白阳性表达22例(58％),FHIT mRNA异常表达为21例(56％)。转染有外源性FHIT的1990细胞(1990 pFHIT)Bax表达阳性率有升高,Bcl-2/Bax比值下降。结论 Bax、Bcl-2及FHIT都通过调控凋亡参与胰腺癌的病理过程。FHIT诱导凋亡可能与Bax上调表达有关。
Objective To study the expression of Bax, Bcl-2 protein and FHIT mRNA in pancreatic carcinoma and explore the significance of their expression in the process of pancreatic carcinoma. Methods Bax, Bcl-2 protein was detected by immunohistochemistry of avidin-biotin complex, and FHIT mRNA was checked by RT-PCR. Results The positive rate of Bax and Bcl-2 protein was 45% and 58%, respectively, in 38 cases of pancreatic carcinoma, and the negative expressing rate of FHIT mRNA was 56%. Immunohistochemical study revealed that the expression of Bax was increased after 1990 cell line transfected with FHIT gene (named as 1990 pFHIT cells). Conclusions Bax,Bcl-2 and FHIT are involved in the development of pancreatic carcinoma by adjusting the path of apoptosis, and the apoptosis induced by FHIT may be related to Bax protein.
出处
《胰腺病学》
2003年第1期30-32,共3页
Chinese JOurnal of Pancreatology
