摘要
本研究以大红泡、黑莓和掌叶覆盆子3种树莓品种为试材,测定其不同发育时期(花期Ⅰ、Ⅱ、Ⅲ和Ⅳ),不同时间段(8:30、10:30、12:30、14:30和16:30),不同干燥条件(自然干燥法和硅胶干燥法)及不同贮藏条件(4和20℃)下的花粉活力,同时测定树莓花粉不同发育时期的柱头可授性,以筛选出适宜的花粉采集、处理时间及贮藏条件。结果表明,树莓最佳花粉采集期为花期Ⅲ,杂交的最佳授粉期为花期Ⅲ,最佳去雄期为花期Ⅰ和Ⅱ;正午间树莓花粉活力均有所下降,最佳采粉时间段是12:30—14:30;硅胶干燥24 h处理后其花粉活力保存较佳;大红泡、黑莓和掌叶覆盆子含水量分别为20.59%、14.67%和15.36%时耐贮藏性较好,在-20℃贮藏条件下能贮藏90 d。研究结果为树莓杂交育种工作提供参考。
In this study,3 species of raspberry were used as test materials,such as Rubus eustephanus Focke ex Diels,Rubus fruticosus Pollich,and Rubus chingii Hu.Pollen viability at different development stages(flowering stageⅠ,Ⅱ,Ⅲ,Ⅳ),different time periods(8:30,10:30,12:30,14:30,16:30),different drying conditions(natural drying method and silica gel drying method),and different storage conditions(4 and 20℃)were measured.The stigma receptivity of raspberry pollen at different developmental stages was determined.To screen out the appropriate pollen collection,treatment time,and storage conditions.The results showed that the best pollen collection period of raspberry was flowering stageⅢ,the best pollination period of hybridization was flowering stageⅢ,and the best emasculation period was flowering stageⅠandⅡ.The pollen activity of raspberry decreased at noon,and the best time period was 12:30-14:30.The pollen viability of silica gel was better preserved after drying for 24 h.When the water content of Rubus eustephanus Focke ex Diels,Rubus fruticosus Pollich,and Rubus chingii Hu was 20.59%,14.67%and 15.36%,respectively,the storage resistance was better,and could be stored for 90 days at-20℃.The results provide a reference for the work of hybridization and breeding of raspberry.
作者
官纪元
张群英
陈哲
杨鼎元
李永霞
沈玺龙
GUAN Jiyuan;ZHANG Qunying;CHEN Zhe;YANG Dingyuan;LI Yongxia;SHEN Xilong(Guizhou Botanical Garden,Guiyang 550004,China;Key Laboratory for Biodiversity Conservation in Karst Mountain Area of Southwestern China,National Forestry and Grassland Administration,Guiyang 550004,China)
出处
《安徽农学通报》
2025年第1期119-124,共6页
Anhui Agricultural Science Bulletin
基金
贵州省科技项目“树莓优新品种鉴选及配套栽培研究与示范”(黔科合支撑〔2021〕一般239)
贵州地区树莓抗氧化物质及其活性研究(黔科院J字〔2021〕7号)
贵州省科技计划项目(黔科合服企〔2021〕004-1)。
关键词
树莓
花粉活力
柱头可授性
低温贮藏
raspberry
pollen vitality
stigma receptivity
low temperature storage
