摘要
DNA或RNA与聚阳离子形成的复合物被广泛用作非病毒类基因载体。由于静电相互作用的高强度和长程性,在溶液中形成的DNA/RNA复合物往往受动力学控制,其结构随着时间会进一步变化。本文总结了这些年来DNA/RNA与聚阳离子复合形成的复合物动力学研究进展,并提出影响复合物的因素主要有两个:近程静电吸引力和远程静电排斥力之间的竞争,以及复合物内、复合物之间的歧化。揭示DNA/RNA与聚阳离子的复合机理,对于制备具有特定结构和功能的基因载体具有重要意义。
The complexes formed by DNA or RNA interacting with polycations have been widely used as non-viral gene vectors.Owing to the strong and long-ranged electrostatic interaction,the complex is usually controlled by kinetics and its structure evolves with time.This review summarized the recent studies on kinetics of the complexation between DNA/RNA with polycations.We proposed that the major factors determining the kinetics of complexation were the balance between the long-ranged electrostatic repulsion force and the short-ranged electrostatic attraction forces,as well as the intra-and intercomplex disproportionation rate.The disclosure of the complexation mechanism is helpful in developing high performance gene delivery vectors.
出处
《高分子通报》
CAS
CSCD
北大核心
2014年第8期34-43,共10页
Polymer Bulletin
基金
国家自然科学基金委资助项目(#21174007)
