摘要
为了解白介素 1β(IL 1β)、肿瘤坏死因子α(TNF α)对大鼠肝星状细胞间质胶原酶 (MMP13 )基因表达的影响 ,在培养的肝星状细胞系中加入IL 1β、TNF α,于不同的时间点收集细胞 ,提取总RNA ,用逆转录定量PCR方法测定MMP13 的基因表达水平。结果表明 ,IL 1β组肝星状细胞MMP13 基因表达水平在 8、2 4、4 8h三个时间点均明显高于对照组 ,2 4h达高峰 ,为对照组的 3倍 ;TNF α组肝星状细胞MMP13 的基因表达水平在 8、2 4、4 8、72h均明显高于对照组 ,4 8h达高峰 ,为对照组的 4倍。上述结果提示IL 1β、TNF α可增强肝星状细胞MMP13
In order to investigate the influence of interleukin 1β(IL 1β) and tumor necrosis factor α (TNF α) on gene expression of rat interstitial collagenase (MMP 13 ) in hepatic stellate cells, cells of a hepatic stellate cell line (HSC T6) were cultured in medium containing IL 1β or TNF α for different incubation periods. Total RNA of HSC was extracted and MMP 13 gene expression levels were measured by reverse transcription polymerase chain reaction. The results showed that MMP 13 gene expression level of the IL 1β group was much higher than that of the control group at 8h, 24h and 48h after exposure to IL 1β. The maximal value was 3 fold of the control group and was at 24h . MMP 13 gene expression level of the TNF α group was also significantly higher than that of control group at 8h, 24h, 48h, 72h, and the maximum nalue was 4 fold of the control group at 48h. These results indicated that IL 1β and TNF α might increase the gene expression of MMP 13 in hepatic stellate cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第11期999-1000,共2页
Medical Journal of Chinese People's Liberation Army
基金
军队"十五"杰出人才基金课题 (编号 0 1J0 0 2 )
