摘要
建立了一种高效液相色谱(HPLC)法同时测定油橄榄中14种抗氧化活性成分(羟基酪醇、酪醇、绿原酸、香草酸、咖啡酸、阿魏酸、毛蕊花糖苷、木犀草苷、芦丁、橄榄苦苷、槲皮素、木犀草素、山奈酚、芹菜素)含量。样品用体积分数70%甲醇水溶液超声提取,以甲醇、体积分数0.1%磷酸水溶液为流动相,梯度洗脱,经InfinityLab Poroshell 120 EC-C18(150 mm×4.6 mm×4μm)色谱柱分离,外标法定量。结果表明,14种化学成分的质量浓度在1~100 mg/L内与其相对峰面积百分比呈良好的线性关系(R2>0.9998)。样品加标回收率为95.3%~107.4%;相对标准偏差(RSD)均小于1.6%(n=6)。该方法具有精密度高、重复性好等优点,能满足油橄榄中14种化合物定量检测的要求,以供对油橄榄进行原料管控和质量评估。
A high performance liquid chromatography(HPLC)method was established to determine the contents of 14 antioxidant ingredients(hydroxytysol,tyrosol,chlorogenic acid,vanilic acid,caffeic acid,ferinic acid,acteoside,luteolin,rutinose,oleuropein,quercetin,luteolin,kaempferol,apigenin)in Olea europaea.Samples were extracted by sonication with 70%(in volume fraction)methanol water solution,gradient eluted with methanol,0.1%(in volume fraction)phosphate acid solution as the mobile phase,separated by InfinityLab Poroshell 120 EC-C18(150 mm×4.6 mm×4μm)column,and quantified by external standard.In the range of 1-100 mg/L,the mass concentration of 14 chemical components had a good linear relationship with their peak area(R2>0.9998).The recovery rates were 95.3%-107.4%,the relative standard deviations(RSDs)were less than 1.6%(n=6).This method has the advantages of high precision and good repeatability,and can meet the requirements of quantitative detection of 14 compounds in Olea europaea,for raw material control and quality evaluation of Olea europaea.
作者
潘丽静
陈韦韦
袁旻嘉
PAN Lijing;CHEN Weiwei;YUAN Minjia(Shanghai Qiran Biotechnology Co.,Ltd.,Shanghai 201700,China;Shanghai Jinjia Technology Co.,Ltd.,Shanghai 201210,China)
出处
《香料香精化妆品》
CAS
2024年第6期21-25,89,共6页
Flavour Fragrance Cosmetics
关键词
高效液相色谱
油橄榄
抗氧化成分
测定
原料
high performance liquid chromatography(HPLC)
Olea europaea
antioxidant composition
determination raw material
