摘要
建立了HPLC法同时测定油橄榄叶抗糖尿病有效部位中的5种多酚类化合物含量。优选的色谱条件为:色谱柱:Sinochrom ODS-BP柱(250×4.6 mm,5μm);DAD检测器;检测波长:230 nm;流速:1.0 mL/min;柱温:30℃;进样量:10μL;流动相:水(A)+甲醇(B),梯度洗脱:0~5 min,30%B;5~20 min,30%B^52%B;20~25 min,52%B^50%B。在优选的色谱条件下,5种多酚类化合物能够得到较好的分离。日内RSD为1.4%~2.6%,日间RSD为0.8%~2.8%,回收率为97.1%~113.2%。方法可用于测定油橄榄叶及其抗糖尿病有效部位中的5种多酚类化合物的含量。
A high performance liquid chromatographic（HPLC） method was developed to determine the five polyphenolic substances in the anti-diabetic effective fraction from the leaves of Olea europaea simultaneously.After optimized the separation condition,the chromatographic condition were as follows：Sinochrom ODS-BP column（250 mm × 4.6 mm,5 μm）,the flow rate of 1 mL/min,the injection volume of 10 μL,and the detection wavelength of 230 nm were used for the separation of the five polyphenolic substances in the leaves of O.europaea within 30 min and the detection temperature was set at 30℃.The linear gradient of deionized water（A） and methanol（B） was set as follows：0 ～5 min,30%B;5 ～20 min,30%B ～52%B;20 ～25 min,52%B～ 50% B.The results show that the five polyphenols were separated well with the intra-day and inter-day precisions（RSD%） for the analytes ranging from 1.4% to 2.6% and from 0.8% to 2.8%,respectively.Their recoveries were from 97.09% to 113.2%.It is a reliable method for rapid,accurate separation and determination of the five polyphenolic substances in the anti-diabetic effective fraction prepared from leaves of Olea europaea L.
出处
《分析试验室》
CAS
CSCD
北大核心
2014年第7期766-770,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金项目(20974116)资助
关键词
高效液相色谱
抗糖尿病
油橄榄叶
多酚
High performance liquid chromatography（HPLC）
Anti-diabetic activity
Olea europaea L.
Polyphenol
