摘要
目的针对丙酮酸假性减低的临床个案,梳理分析前干扰因素对丙酮酸检测结果的影响,优化检验路径,确保检测质量。方法分别使用分离胶采集管和肝素抗凝管采集血液样本,分析不同样本周转时间(TAT)、冷藏(2~8℃)和室温保存样本的丙酮酸变化率。结果分离胶采集管对比肝素抗凝管,在未离心条件下放置30min和60min时丙酮酸变化率差异均具有统计学意义(P<0.05);在离心条件下放置30min、60min和120min时丙酮酸变化率差异均具有统计学意义(P<0.05)。未离心的分离胶采集管样本保存在室温对比冷藏时丙酮酸变化率差异具有统计学意义(P<0.05)。结论丙酮酸检测结果假性减低的影响因素主要包括采血管种类、样本周转时间和保存温度。推荐使用分离胶采集管,室温保存在30min内进行离心检测,丙酮酸检测结果更稳定。
Objective To identify and evaluate the potential interference factors on pyruvate detection results before analysis in view of the clinical cases of false decrease of pyruvate,and to optimize the detection pipeline to ensure the detection quality.Methods Blood samples were collected by separating gel collection tube and heparin anticoagulant tube respectively,and the change rate of pyruvate for different sample turnover time(TAT),cold storage and room temperature storage conditions were analyzed.Results There was a statistically significant difference in the change rate of pyruvate between heparin anticoagulant tube and separating gel collection tube at 30 minutes and 60 minutes without centrifugation(P<0.05).There was a statistically significant difference in the change rate of pyruvate between heparin anticoagulant tube and separating gel collection tube at 30 minutes,60 minutes and 120 minutes under centrifugal conditions(P<0.05).There was a statistically significant difference between cold and room temperature storage conditions in the non-centrifuged separated gel blood collection tubes(P<0.05).Conclusion The influencing factors of false decrease in pyruvate detection results mainly include blood collection tube types,sample turnover time and storage temperature conditions.It was recommended to use separation gel to collect blood tubes,and centrifuge them within 30 minutes after storage at room temperature,and the test results are expected to be more stable with these optimization steps.
作者
郑荣
冯杰
石纯倩
高艳红
ZHENG Rong;FENG Jie;SHI Chunqian;GAO Yanhong(Clinical Laboratory Department,the First Medical Center of the PLA General Hospital,Beijing 100853,China;Xingtai General Hospital of North China Medical and Health Group,Xingtai 054000,China)
出处
《标记免疫分析与临床》
CAS
2024年第11期2047-2050,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家重点研发计划课题(编号:2017YFF0205401)。
关键词
采血管
样本周转时间
温度
丙酮酸变化率
Blood collection tube
Sample turnover time
Temperature
Change rate of pyruvate
