摘要
特异根系分泌物-三丙二醇单甲醚(TPM)在玉米花生间作系统下明显增多,探明其对花生植株生长和代谢生理的影响,可为探索调控花生生长发育的有效物质,促进花生提质增产提供参考。本研究以青花6号花生品种为试验材料,以等体积无菌水添加为对照(CK),研究了3种TPM添加梯度下(T1:10.23μL/盆、T2:20.46μL/盆、T3:40.92μL/盆)花生植株干物质积累、最大光化学效率、碳氮代谢酶活及抗氧化特性。研究表明:与CK相比,T1和T3处理显著降低了花生植株地上部干物重,所有TPM添加处理均显著降低了花生植株地下部干物重和PSⅡ最大光化学效率(Fv/Fm);TPM添加处理显著提高了花生功能叶片碳氮代谢酶活性,其中,T1处理下硝酸还原酶(NR)、蔗糖合成酶(SS)以及磷酸蔗糖合成酶(SPS)活性较CK提升6.49%~203.24%,T3处理下谷氨酰胺合成酶(GS)活性较CK显著提升39.81%;TPM添加对花生植株产生胁迫影响,各添加处理功能叶片丙二醛(MDA)含量均显著增加,较CK提升17.32%~34.65%,T3处理下超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性较CK分别降低75.82%和75.74%,T1处理下过氧化物酶(POD)活性较CK显著降低30.43%;通过组织化学定位看出,T3处理明显诱导了花生植株功能叶片H2O2和O2-的积累,其中O2-含量较CK显著增加了83.39%。综上可见,TPM会抑制花生植株尤其是地下部生长,降低叶片潜在最大光合能力(Fv/Fm)并抑制SOD、CAT、POD等抗氧化酶活性,但花生植株可通过提升NR、SS、SPS和GS等碳氮代谢酶活性来降低TPM造成的损伤;本研究认为,TPM不适用于花生生产。
The specific root exudate,tripropylene glycol monomethyl ether(TPM),was significantly increased under the maize and peanut intercropping system.Exploring its effects on the growth and metabolic physiology of peanut plants could provide reference for exploring effective substances regulating the growth and development of peanut and promoting the quality and yield improvement of peanut.In this study,Qinghua No.6 peanut variety was used as experimental material,and the same volume of sterile water was added as control(CK).The dry matter accu⁃mulation,maximum photochemical efficiency,carbon and nitrogen metabolism enzyme activity and antioxidant properties of peanut plants under three tripropylene glycol monomethyl ether(TPM)adding gradient(T1:10.23μL/pot,T2:20.46μL/pot,T3:40.92μL/pot)were studied.The results showed that,compared with CK,T1 and T3 signif⁃icantly reduced the above-ground dry matter weight of peanut plants.All TPM treatments significantly reduced the dry matter weight of underground part and maximum photochemical efficiency(Fv/Fm)of PSⅡ.TPM supplementa⁃tion significantly increased the activity of carbon and nitrogen metabolizing enzymes in peanut functional leaves,for detail,the activities of nitrate reductase(NR),sucrose synthetase(SS)and phosphate sucrose synthetase(SPS)in T1 treatment were increased by 6.49%-203.24%compared with CK,and Glutamine synthetase(GS)activity in T3 treatment was significantly increased by 39.81%compared with CK.The supplementation of TPM had stress effect on peanut plants.The content of malondialdehyde(MDA)was significantly increased by 17.32%-34.65%compared with CK.The activities of superoxide dismutase(SOD)and catalase(CAT)under T3 treatment were decreased by 75.82%and 75.74%compared with CK,respectively.The peroxidase(POD)activity under T1 treatment was signifi⁃cantly decreased by 30.43%compared with CK.According to the histochemical localization,T3 treatment signifi⁃cantly induced the accumulation of H2O2 and O2-in peanut plant functional leaves,and the content of O2-was signif⁃icantly increased by 83.39%compared with CK.In conclusion,TPM can inhibit the growth of peanut plants,espe⁃cially the underground part,reduce the potential maximum photosynthetic capacity(Fv/Fm)of leaves and inhibit the activities of antioxidant enzymes such as SOD,CAT and POD.However,peanut plants can reduce the damage caused by TPM by improving the activities of carbon and nitrogen metabolic enzymes such as NR,SS,SPS and GS.In this study,TPM is not suitable for peanut production.
作者
刘燕
蔺益民
黄明明
司彤
于晓娜
张晓军
邹晓霞
LIU Yan;LIN Yi-min;HUANG Ming-ming;SI Tong;YU Xiao-na;ZHANG Xiao-jun;ZOU Xiao-xia(College of Agronomy,Qingdao Agricultural University/Shandong Key Lab.of Dry Farming Techniques,Qingdao 266109,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2024年第6期1320-1328,共9页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(42107376)
国家现代农业产业技术体系(CARS-13)
青岛农业大学研究生创新计划(QNYCX22015)。
关键词
花生
三丙二醇单甲醚
干物质积累
碳氮代谢酶
抗氧化特性
peanut
tripropylene glycol monomethyl ether
dry matter quality
carbon and nitrogen metaboliz⁃ing enzyme
antioxidant properties
