摘要
【目的】TIFY基因在植物生长发育过程中发挥重要的调节作用,对丝瓜TIFY基因家族成员进行鉴定,筛选果实中高表达的成员,并对其进行克隆和组织表达分析,为阐明其生物学功能奠定基础。【方法】通过生物信息学方法对丝瓜TIFY基因家族成员进行鉴定,基于转录组分析筛选果实中高表达的成员,基于RT-PCR技术对其进行克隆,基于qRT-PCR研究其组织表达。【结果】丝瓜基因组中包含17个TIFY基因,不均匀地分布于9条染色体上,CDS序列全长297~1353 bp,可分为4个亚家族;LcTIFY2、LcTIFY7、LcTIFY11和LcTIFY13在丝瓜果实中的表达量显著高于其他成员;克隆了其CDS序列,分别具有3,5,6,2个外显子;上述4个基因均在丝瓜果皮中的表达量最高,此外,LcTIFY7和LcTIFY13在生殖器官中的表达量显著高于营养器官;LcTIFY11在雌花蕾和子房中的表达量显著高于雄花蕾。【结论】丝瓜TIFY家族成员LcTIFY2、LcTIFY7、LcTIFY11和LcTIFY13可能在其生殖生长和果实发育过程中发挥作用。
[Objective]TIFY gene are involved in the regulation of growth and development in plants.This experiment involves the identification of the TIFY family members in Luffa cylindrica,selecting the highly expressed members in fruits,and further cloning and investigating their expression patterns in different tissues,in order to provide basis for the functional identification of these genes.[Methods]Bioinformatics methods were used to identify the TIFY gene family members in L.cylindrica.Transcriptome analysis was performed to screen the highly expressed members in L.cylindrica fruits,followed by cloning genes using RT-PCR.Tissue expression was analyzed using qRT-PCR.[Results]L.cylindrica genome contains 17 TIFY genes,which were unevenly distributed across 9 chromosomes,with CDS lengths ranging from 297 bp to 1353 bp,classifing into four subfamilies.Transcriptome analysis revealed that LcTIFY2,LcTIFY7,LcTIFY11,and LcTIFY13 were highly expressed in L.cylindrica fruits.The CDS sequences of these genes were cloned,comprising 3,5,6,and 2 exons,respectively.Tissue expres sion analysis indicated that all four genes had the highest expression in the peel of L.cylindrica fruits.Additionally,LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs,while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries.[Conclusion]The TIFY family members LcTIFY2,LcTIFY7,LcTIFY11 and LcTIFY13 may play roles in reproduc-tive growth and fruit development in L.cylindrica.
作者
刘同金
刘振洋
班秋妍
周露
崔群香
LIU Tongjin;LIU Zhenyang;BAN Qiuyan;ZHOU Lu;CUI Qunxiang(College of Horticulture,Jinling Institute of Technology,Nanjing 210038,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第11期1752-1759,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家级大学生创新训练计划项目(202313573033Z)
南京市品种选育专项项目(202210020)
金陵科技学院高层次人才科研启动项目(jit-b-202009)
金陵科技学院科研基金孵化项目(jit-fhxm-202113)。
关键词
丝瓜
TIFY基因表达
果实发育
Luffa cylindrica
gene expression of TIFY
fruit development
