摘要
成蛋白(formins)在细胞骨架的调控中起着关键作用,并通过该过程参与细胞的动态调控。研究成蛋白对进一步了解真核细胞的生长和形态变化具有重要意义。但玉米(Zea mays)作为一种重要的农作物,其成蛋白家族并未被系统性鉴定以及分析。因此,本文通过对两种模式植物水稻(Oryza sativa)和拟南芥(Arabidopsis thaliana)的成蛋白进行同源性分析,鉴定出玉米中22个成蛋白家族成员,其氨基酸数目为300~1699 aa,蛋白分子量为36376.32~184309.82 kDa,等电点为6.88~9.76。基于它们的结构域组成,这些成员可以分为16个植物Ⅰ类成蛋白,6个植物Ⅱ类成蛋白。亚细胞定位和蛋白互作网络分析结果表明,玉米成蛋白主要定位在细胞质膜上,且主要与抑制蛋白(profilin)以及ROP(rho-related GTPases from plants)蛋白互作。GO功能富集网络分析表明,玉米成蛋白主要参与细胞骨架的组成以及介导肌动蛋白聚合过程。顺式作用元件分析结果表明,玉米成蛋白基因受生长素、赤霉素等激素以及光照、昼夜节律、温度等环境因子的调控,并且都存在厌氧诱导相关顺式调节元件。基因表达模式分析表明,玉米成蛋白基因在玉米根部的表达水平更高,暗示成蛋白基因可能在玉米根部起着关键作用。共线性分析表明,水稻和玉米具有较高的共线性,说明两种植物基因组进化的相似性。综上所述,该研究为未来对玉米成蛋白的研究提供了启示,并为进一步研究成蛋白在玉米中的功能提供了方向。
Formins,who play a crucial role in regulating the cytoskeleton,are involved in the dynamic regulation of cell morphology.The research on the function and signal pathways of Formins are meaningful for exploring eukaryotic cells’growth and morphological changes.But the Formin family of Zea mays which is one of the most important crop plants,has not been characterized yet.Therefore,in this study,22 Formin family members in maize have been identified by homology analysis with Formins from the two model plants(rice and Arabidopsis).The numbers of amino acids ranged from 300 to 1699 aa,the molecular weight of the proteins ranged from 36376.32 to 184309.82 kDa,and the isoelectric points ranged from 6.88 to 9.76.Based on their domain composition,these maize Formins could be classified as 16 type I Formins and 6 typeⅡFormins.The analyses of the subcellular localization and protein-protein interaction network showed that maize Formin proteins were mainly located in the plasma membrane,and most of them interacted with profilin and ROP protein.Go function enrichment analysis of the network showed that Formin proteins were mainly involved in the composition of the cytoskeleton and the process of actin-binding.The result of cis-acting element analysis showed that maize Formin genes are regulated by hormones including auxin,gibberellin as well as environmental factor like light,circadian rhythm,temperature and so on.Intriguingly,they all had cis-regulatory elements related to anaerobic induction.The expression pattern analysis showed that maize Formin genes were highly expressed in the root,implying that they might function in the roots development or activities.Collinearity analysis showed that rice and maize shared high collinearity,suggesting that the similarity of genome evolution between the two species.In summary,the study sheds light on future research on maize Formin family and provides a direction for further research on the function of Formin in corn.
作者
杜志烨
李斌
石杨
姜南
陈稷
黄进
Du Zhiye;Li Bin;Shi Yang;Jiang Nan;Chen Ji;Huang Jin(College of Ecology and Environment,Chengdu University of Technology,Chengdu,610059,China;College of Agronomy,Sichuan Agricultural University,Chengdu,611130)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第7期1505-1520,共16页
Genomics and Applied Biology
基金
国家自然科学基金项目(31870383)资助
关键词
生物信息学
成蛋白
玉米
蛋白互作
共线性分析
Bioinformatics
Formin
Maize
Protein-protein interaction
Collinearity analysis
