摘要
目的探讨三棱、莪术提取物对骨关节炎大鼠软骨细胞损伤以及NADPH氧化酶2(NOX2)/活性氧(ROS)/核因子-κB(NF-κB)信号通路的影响。方法将50只大鼠随机分为假手术组、模型组及三棱、莪术提取物低、中、高剂量组,每组10只。除假手术组外,构建膝关节炎软组织损伤的大鼠模型,建模后第2天开始给药,三棱、莪术提取物低、中、高剂量组大鼠灌胃三棱、莪术提取物5、10、20 g/kg,假手术组和模型组大鼠灌胃等量0.9%氯化钠溶液,每天1次,连续灌胃给药20 d。观察各组大鼠膝关节行为学、骨代谢指标、血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)水平、炎性因子以及膝关节组织中NOX2和NF-κB蛋白表达水平。结果与模型组比较,三棱、莪术提取物低、中、高剂量组小鼠行为异常评分、软骨寡聚基质蛋白(COMP)、MDA、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、NOX2和NF-κB水平均逐渐降低(均P<0.05),蛋白聚糖、SOD、GSH和白细胞介素-10(IL-10)水平均逐渐升高(均P<0.05),且具有剂量相关性。结论三棱、莪术提取物可有效改善大鼠骨关节炎软骨损伤,可能与抑制NOX2/ROS/NF-κB信号通路活性有关。
ObjectiveTo investigate the effects of extracts of Rhizoma Sparganii and Rhizoma Curcumae on cartilage damage in osteoarthritis rats and NADPH oxidase 2(NOX2)/reactive oxygen species(ROS)/nuclear factor-κB(NF-κB)signaling pathways.MethodsRats(50 cases)were divided into the sham group,and model group,as well as the low,medium,and high dose groups of extracts of Rhizoma Sparganii and Rhizoma Curcumae,with 10 rats in each group.Except for sham group,the rat model of cartilage damage in knee osteoarthritis was established.On the second day after modeling,the rats in the low,medium,and high dose groups received intragastric extracts perfusion of Rhizoma Sparganii and Rhizoma Curcumae at the doses of 5,10,and 20 g/kg respectively.The rats in the sham and model groups received intragastric equivalent 0.9%sodium chloride solution perfusion,once daily,for 20 days by continuous administration.The knee joint behavior,bone metabolism indicators,serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH)levels,inflammatory factors,NOX2,and NF-κB levels of each group were observed.ResultsCompared with the model group,the behavioral abnormality scores,cartilage oligomeric matrix protein(COMP),MDA,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),NOX2,and NF-κB levels in the low,medium,and high dose groups were all gradually decreased(all P<0.05),while proteoglycan,SOD,GSH,and interleukin-10(IL-10)levels in the low,medium,and high dose groups were all gradually increased(all P<0.05),and it was dose-dependent.Conclusions Rhizoma Sparganii and Rhizoma Curcumae extracts can effectively improve cartilage damage in osteoarthritis rats,and it may be related to the inhibition of the NOX2/ROS/NF-κB signaling pathway.
作者
马金超
陈明浩
姜春乾
王阳
刘兴国
李永全
Ma Jinchao;Chen Minghao;Jiang Chunqian;Wang Yang;Liu Xingguo;Li Yongquan(Department of Orthopedics,Tianjin TEDA Hospital,Tianjin 300457,China)
出处
《国际生物医学工程杂志》
CAS
2024年第3期242-246,共5页
International Journal of Biomedical Engineering
关键词
骨关节炎
三棱提取物
莪术提取物
NADPH氧化酶2
活性氧
核因子-ΚB
信号通路
Osteoarthritis
Rhizoma Sparganii extract
Rhizoma Curcumae extract
NADPH oxidase 2
Reactive oxygen species
Nuclear factor-κB
Signaling pathway
