摘要
目的探讨三十六荡坎蛤散对哮喘缓解期小鼠气道炎症的作用。方法将78只BALB/c小鼠随机分为空白组、模型组、地塞米松组(0.5mg/kg)和三十六荡坎蛤散低、中、高剂量组(9.3、18.6、37.2g/kg)。除空白组外,其余各组均采用腹腔注射卵清蛋白(OVA)和氢氧化铝混合液致敏,并激发建立小鼠哮喘缓解期模型,造模第77天给药干预,连续28d。采用HE染色观察小鼠肺组织病理改变,ELISA法检测血清和肺泡灌洗液(BALF)中IL-1β、IL-6、TNF-α、HMGB1、NF-κB水平,Westernblot法检测肺组织TLR4、MyD88、NF-κBp65、p-NF-κBp65蛋白表达。结果与模型组比较,各给药组哮喘缓解期小鼠肺组织病理性损伤和炎症反应明显减轻,血清和BALF中IL-1β、IL-6、TNF-α、HMGB1、NF-κB水平均降低(P<0.01),肺组织TLR4、MyD88、p-NF-κB p65/NF-κB p65蛋白表达均降低(P<0.01),其中三十六荡坎蛤散中剂量组效果较佳,仅次于地塞米松组。结论三十六荡坎蛤散对哮喘缓解期小鼠气道炎症具有良好的抑制作用,其机制可能与抑制HMGB1/TLR4/MyD88信号通路活化有关。
AIM To investigate the effects of Sanshiliudang Kange Powder on airway inflammation in bronchial asthma remission of mice.METHODS Seventy-eight BALB/c mice were randomly divided into the blank group,the model group,the dexamethasone group(0.5 mg/kg)and the low-dose,medium-dose and high-dose Sanshiliudang Kange Powder groups(9.3,18.6 and 37.2 g/kg).Except for those of the blank group,the mice of the other groups were sensitized by intraperitoneal injection of ovalbumin(OVA)and aluminum hydroxide mixture,and induced into models of asthma remission followed by 28 days consecutive corresponding drug administration which was initiated on the 77th day of modeling.The mice had their pulmonary pathological changes observed by HE staining;their levels of IL-1β,IL-6,TNF-α,HMGB1 and NF-κB in serum and alveolar fluid determined by ELISA;and their pulmonary protein expressions of TLR4,MyD88,NF-κB p65 and p-NF-κB p65 determined by Western blot.RESULTS Compared with the model group,each group intervened with a drug displayed significantly reduced pulmonary pathological injury and inflammation;decreased levels of IL-1β,IL-6,TNF-α,HMGB1,NF-κB in serum and BALF(P<0.01);and decreased pulmonary protein expressions of TLR4,MyD88,p-NF-κB p65/NF-κB p65(P<0.01).Generally,the medium-dose Sanshiliudang Kange Powder groupdemonstrated a very good efficacy,which was only inferior to that of the dexamethasone group.CONCLUSION Sanshiliudang Kange Powder has a good inhibitory effect on airway inflammation in bronchial asthma remission of mice,and its mechanism may be related to the inhibited activation of HMGB1/TLR4/MyD88 signal pathway.
作者
杨丽琴
吴玉苗
黄柳青
雍亚云
李伟伟
YANG Li-qin;WU Yu-miao;HUANG Liu-qing;YONG Ya-yun;LI Wei-wei(Guangxi University of Chinese Medicine,Nanning 530200,China;The First Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530023,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第7期2196-2201,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81860867)
中医药人才队伍建设专项经费-广西名中医工作室建设项目(2021022-001-08)
广西中医药大学2021年校级博士研究生科研创新项目(YCBXJ2021017)
广西中医药大学校级科研项目(2022MS011)。
