摘要
研究桂林会仙湿地来源真菌Talaromyces sp.W21的化学成分及其抗炎活性。综合运用多种柱色谱、现代波谱技术和理化性质对Talaromyces sp.W21发酵提取物中的化学成分进行分离、纯化和结构鉴定,并从中分离得到10个化合物,分别鉴定为cytospone K(1)、(3 S,4 R,5 R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2)、lasdiplactone(3)、leptosphaerone C(4)、annularin A(5)、annularin K(6)、cladobotrin V(7)、2-(2′S-hydroxypropyl)-5-methyl-7-hydroxychromone(8)、bacillisporin A(9)、bacillisporin B(10)。其中,化合物1为新的α-吡喃酮类化合物,化合物2~8为首次从Talaromyces sp.中分离获得。抗炎活性实验结果表明化合物9和10对LPS诱导的RAW 264.7释放NO具有一定的抑制作用,其IC 50分别为38.90±0.79、35.02±1.03μmol/L。
This study aims to investigate the chemical constituents and anti-inflammatory activity of Talaromyces sp.W21 from the Huixian wetland in Guilin.A new α-pyrone derivative,namely cytospone K(1),together with nine known compounds,namely(3 S,4 R,5 R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2),lasdiplactone(3),leptosphaerone C(4),annularin A(5),annularin K(6),cladobotrin V(7),2-(2′S-hydroxypropyl)-5-methyl-7-hydroxychromone(8),bacillisporin A(9)and bacillisporin B(10)were isolated and purified from cultures of Talaromyces sp.W21 by using various column chromatography.Their structures were identified by modern spectroscopic techniques and physicochemical properties.Compound 1 was a new α-pyranone derivative,and compounds 2-8 were isolated from the Talaromyces sp.for the first time.The anti-inflammatory activity assay showed that compounds 9 and 10 have a moderate inhibition of NO production induced by LPS in RAW 264.7 cells,with IC 50 values of 38.90±0.79 and 35.02±1.03μmol/L respectively.
作者
梁琳
袁玺
冼小雅
周先丽
王力生
邝彤东
梁成钦
LIANG Lin;YUAN Xi;XIAN Xiao-ya;ZHOU Xian-li;WANG Li-sheng;KUANG Tong-dong;LIANG Cheng-qin(School of Pharmacy,Guilin Medical University;School of Intelligent Medicine&Biotechnology,Guilin Medical University;Guangxi Key Laboratory of Diabetic Systems Medicine,Guilin Medical University,Guilin 541199,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第7期1175-1181,共7页
Natural Product Research and Development
基金
国家自然科学基金(81860621)。
关键词
湿地真菌
化学成分
α-吡喃酮
抗炎活性
wetland fungus
chemical constituents
α-pyrone derivative
anti-inflammatory activity
