摘要
目的制备靶向肝癌的MRI纳米探针IR-PEG-FA-Gd(IPFG),评估其近红外光热转化性能,探讨其光热治疗肝癌的效果。方法用物理螯合法将Gd3+螯合至IR-PEG-FA纳米粒子中,制备靶向肝癌的MRI纳米探针IPFG;使用808nm激光器监测其体内外光热升温性能;构建原位肝癌小鼠模型,利用IVIS成像和MRI监测IPFG光热治疗肝癌的效果。结果在808nm激光照射下,IPFG溶液在4min内温度可达87.3℃;皮下瘤模型中IPFG组3min内瘤体温度达到48.0℃。即便覆盖3mm鸡胸肉组织模拟生物环境,IPFG溶液仍能在4 min内升温至47.3℃;经光热治疗后,IPFG组肿瘤信号几乎消失,显著优于IPG组及对照组,且IPFG组MRI成像分辨率更高。结论IPFG具有良好的近红外光热转换效能,其在肝癌光热治疗中展现出优异的MRI成像和光热治疗效果,为肝癌的精准治疗提供了新的策略。
Objective To prepare IR-PEG-FA-Gd(IPFG),an MRI nanoprobe targeting hepatocellular carcinoma,to evaluate its near-infrared photothermal conversion performance,and to explore its efficacy in photothermal therapy of hepatocellular 3+carcinoma.Methods Gd was chelated into IR-PEG-FA nanoparticles using the physical chelation method,and the MRI nanoprobe IPFG targeting hepatocellular carcinoma was prepared;the MRI nanoprobe IPFG targeting hepatocellular carcinoma was prepared;its ex vivo and in vivo photothermal heating performance was monitored using 808 nm lasers;the in situ hepatocellular carcinoma mouse model was constructed,and the effects of photothermal therapy treatment for hepatocellular carcinoma were monitored by using IVIS imaging and MRI to monitor the photothermal therapy treatment effect of IPFG for hepatocellular carcinoma.Results Under 808 nm laser irradiation,the temperature of IPFG solution could reach 87.3°C within 4 min;the tumor temperature of IPFG group reached 48.0°C within 3 min in the subcutaneous tumor model.Even when covered with 3 mm chicken breast tissue to simulate the biological environment,the IPFG solution could still warm up to 47.3°C within 4 min;after photothermal therapy treatment,the tumor signal almost disappeared in the IPFG group,which was significantly better than that in the IPG group and the control group,and the resolution of MRI imaging was higher in the IPFG group.Conclusion IPFG has good near-infrared photothermal conversion efficacy,and it demonstrates excellent MRI and photothermal treatment effects in liver cancer photothermal therapy,which provides a new strategy for precision treatment of liver cancer.
作者
王雅飞
高琴
李科
徐钰叶
程梓荷
潘奇
WANG Yafei;GAO Qin;LI Ke;XU Yuye;CHENG Zihe;PAN Qi(The Second Clinical Medical College of Xi'an Medical College,Xi'an 710000,China;Department of Medical Imaging,The Second Affiliated Hospital,Xi'an Medical University,Xi'an 710038,China;Institute of Basic and Translational Medicine,Xi'an Medical College,Xi'an 710021,China)
出处
《分子影像学杂志》
2024年第6期569-574,共6页
Journal of Molecular Imaging
基金
陕西省科技厅重点研发项目(2021SF-271)
陕西省教育厅服务地方专项(22JC056)
西安市科技局一般项目(24YXYJ0188)西安医学院第二附属医院院级课题(24KY0102、24KY0106、24KY0121)
大学生创新创业计划训练项目(202311840014)。
关键词
肝癌
纳米探针
磁共振成像
光热治疗
liver cancer
nanoprobe
magnetic resonance imaging
photothermal therapy
