摘要
目的:构建负载二氧化锰(MnO_(2))纳米颗粒的可得然(Cur)复合水凝胶MnO_(2)@Cur(简称MGel),研究其对黑色素瘤B16-F10细胞的杀伤效果。方法:采用热诱导法制备Cur水凝胶(Gel),物理负载MnO_(2)构建MGel,表征其宏观和微观形貌,检测其机械性能、降解性能以及光热转换性能等理化性能,并研究其联合PTT对小鼠皮肤黑色素瘤B16-F10细胞的光热杀伤效果。结果:MGel具有优异的机械和可降解性能,抗拉伸强度达(127.97±3.60)kPa、抗压缩强度达(151.44±5.23)kPa,28 d降解率约58.17%。MGel负载MnO_(2)纳米片(粒径约180 nm)获得优异的光热转换性能,负载1.0 mg/mL MnO_(2)的MGel在1.0 W/cm^(2)的808 nm NIR光照4 min后到达最高温度50℃。细胞毒性实验和Calcein-AM/PI荧光双染色实验表明,MGel联合PTT有效杀伤B16-F10黑色素瘤细胞,NIR光照使得MGel组细胞存活率降低至(4.68±0.66)%(P<0.0001)。结论:MGel复合水凝胶具备优异的机械性能、可降解性能以及光热转换性能,其联合PTT能有效杀伤肿瘤细胞,可能成为一种有效治疗黑色素瘤的新手段。
Objective:Crudlan(Cur)was loaded with manganese dioxide(MnO_(2))nanoparticle to construct a composite hydrogel MnO_(2)@Cur(abbreviated as MGel)and its killing effect on melanoma B16-F10 cells was studied.Methods:Firstly,Cur hydrogel(Gel)was prepared by thermal induction method,and then MnO_(2)was physically loaded to construct MGel.The macroscopic and microscopic morphology of MGel was characterized and its mechanical properties,degradation properties and photothermal conversion properties were detected.The photothermal killing effect of MGel combined with PTT on mouse cutaneous melanoma B16-F10 cells was studied.Results:MGel is excellent in mechanical strength and degradation performance.Its tensile strength reached(127.97±3.60)kPa,and compressive strength reached(151.44±5.23)kPa;its degradation rate on the 28th day was about 58.17%.MGel obtained excellent photothermal conversion capability after loading MnO_(2)nanosheets with the average particle diameter of about 180 nm.After receiving 808 nm NIR illumination at 1.0 W/cm^(2) for 4 minutes,MGel loaded with 1.0 mg/mL MnO_(2)reached a maximum temperature of 50℃.Cytotoxicity experiments and Calcein-AM/PI fluorescence double staining showed that MGel combined with PTT killed B16-F10 melanoma cells effectively,and NIR illumination reduced the cell survival rate of the MGel group to(4.68±0.66)%(P<0.0001).Conclusion:Composite hydrogel MGel has excellent mechanical strength,degradation performance and photothermal conversion capability.MGel combined with PTT can kill tumor cells effectively,which provides a novel strategy for the treatment of melanoma.
作者
张停琳
吴丽丽
王雨
张转转
周旋
李美桂
严珍珍
丁秀文
陆松伟
陈翠敏
梁浩
张梦亚
高洁
ZHANG Tinglin;WU Lili;WANG Yu;ZHANG Zhuanzhuan;ZHOU Xuan;LI Meigui;YAN Zhenzhen;DING Xiuwen;LU Songwei;CHEN Cuimin;LIANG Hao;ZHANG Mengya;GAO Jie(Clinical Research Unit,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Burn Surgery,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Pharmacy,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Orthopedics,Jinshan Hospital,Hehai University,Shanghai 200540,China;Institute of Translational Medicine,Shanghai University,Shanghai 200040,China;College of Pharmacy,Henan University,Zhengzhou 475004,Henan,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2023年第8期656-664,共9页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金(No.82072051)。
