摘要
目的研究瓜蒌Trichosanthes kirilowii Maxim.干燥成熟果皮的化学成分。方法应用硅胶、Sephadex LH-20凝胶、ODS反相色谱柱以及半制备HPLC等方法对瓜蒌皮水提物进行分离纯化,根据1H-NMR、13 C-NMR和HRMS等波谱学方法对所得化合物进行结构鉴定。结果从瓜蒌皮水提物的乙酸乙酯萃取部位共分离得到12个化合物,其结构鉴定为葫芦素D(1)、葫芦素B(2)、异葫芦素D(3)、fabacein(4)、23,24-二氢葫芦素D(5)、柯伊利素7-O-β-D吡喃葡萄糖苷(6)、blumenol A(7)、对羟基苯甲酸(8)、5-oxymaltol(9)、香草酸(10)、烟酸(11)和3-maleimide-5-oxime(12)。结论化合物4为首次从天然植物中分离得到,化合物3、8、9、11、12为首次从瓜蒌皮中分离得到。
Objective To investigate the chemical components of the dry mature fruit peel of Trichosanthes kirilowii Maxim.Methods Silicagel,Sephadex LH-20,ODS reversed phase column,and semi-preparative HPLC were used for the isolation and purification of the water extract of Trichosanthes kirilowii Maxim.peel,and spectroscopy(1H-NMR,13 C-NMR,and HRMS)was used for the structural identification of the compounds obtained.Results A total of 12 compounds were isolated from the ethyl acetate fraction of the water extract and were structurally identified as cucurbitacin D(1),cucurbitacin B(2),isocucurbitacin D(3),fabacein(4),23,24-dihydrocu-curbitacin D(5),chrysoeriol-7-O-β-D-glucoside(6),blumenol A(7),4-hydroxybenzoicacid(8),5-oxymaltol(9),vanillic acid(10),nicotinic acid(11),and 2-methyl maleimide-5-oxime(12).Conclusion Compound 4 is isolated from a natural product for the first time,and compounds 3,8,9,11,and 12 are isolated from T.kirilowii peel for the first time.
作者
尹嘉敏
王仁中
曾凡俊
刘劲松
黄圣卓
许凤清
吴德玲
YIN Jiamin;WANG Renzhong;ZENG Fanjun;LIU Jinsong;HUANG Shengzhuo;XU Fengqing;WU Deling(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Anhui Province Key Laboratory of Research&Development of Chinese Medicine,Anhui Hefei230012,China;General Administration of Customs(Beijing)International Travel Health Care Center,Beijing 100013,China;Hainan Institute for Tropical Agricultural Resources,Chinese Academy of Tropical Agricultural Sciences,Hainan Haikou 571101,China)
出处
《安徽中医药大学学报》
CAS
2024年第3期104-109,共6页
Journal of Anhui University of Chinese Medicine
基金
安徽中医药大学—潜山市医药健康发展产业科研攻关项目(2021QSHZ03)
安徽省高等学校自然科学研究项目(KJ2021A0594)。
关键词
瓜蒌皮
化学成分
提取分离
结构鉴定
Trichosanthes kirilowii peel
Chemical components
Extraction and isolation
Structural identification
