摘要
目的 探究淫羊藿苷对骨折大鼠骨髓基质干细胞(BMSCs)增殖、成骨分化的影响及可能机制。方法 选取SPF级SD大鼠5只,8周龄,雌雄不限,体重(290±20)g。麻醉大鼠,于右膝髌骨内侧做一切口,暴露股骨髁间凹,在髁内钻孔后,将克氏针插入胫骨髓腔,钢锯横行截断股骨,形成横行骨折,缝合。骨折后2 d,麻醉后断颈处死,剥离股骨,体外分离BMSCs。取第三代BMSCs分为对照组(不做特殊处理),低、中、高剂量组(加入终浓度为1、10、100μmol/L的淫羊藿苷溶液)。检测各组BMSCs增殖情况、碱性磷酸酶(ALP)活性、矿化结节面积、Janus激酶2(JAK2)、信号转导与转录激活因子5(STAT5)、p-STAT5表达情况。结果 与对照组比较,低、中、高剂量组光密度(OD)值、ALP活性、矿化结节面积占比均升高,JAK2、p-STAT5/STAT5均降低(P<0.05);与低剂量组比较,中、高剂量组OD值、ALP活性、矿化结节面积占比均升高,JAK2、p-STAT5/STAT5均降低(P<0.05);与中剂量组比较,高剂量组OD值、ALP活性、矿化结节面积占比升高,JAK2、p-STAT5/STAT5均降低(P<0.05)。结论 淫羊藿苷可能通过抑制JAK2/STAT5通路促进骨折创伤大鼠BMSCs增殖及成骨向分化。
Objective To explore the effect and possible mechanism of icariin on proliferation and osteogenic differentiation of bone marrow stromal cells(BMSCs)in fractured rats.Methods Five SPF-grade SD rats were selected,eight weeks old,male or female,weighing(290±20)g.The rats were anesthetized,and the intercondylar cavity of the femur was exposed.After drilling the condyle,the Kirschner needle was inserted into the tibial pulp cavity,and the femur was amputated with a hacksaw to form a transverse fracture and sutured.Two days after the fracture,the neck was severed after anesthesia,the femur was stripped,and BMSCs were isolated in vitro.The 3rd generation BMSCs were divided into control group(no special treatment),low-,medium-,and high-dose groups(adding icariin solution with final concentration of 1,10,and 100μmol/L).The proliferation,alkaline phosphatase(ALP)activity,mineralized nodule area,Janus kinase 2(JAK2),signal transducer and activator of transcription 5(STAT5),and p-STAT5 expression of BMSCs in each group were detected.Results Compared with control group,the optical density(OD)value,ALP activity,and the proportion of mineralized nodule area of low-,medium-,and high-dose groups were increased,JAK2 and p-STAT5/STAT5 were decreased(P<0.05);compared with low-dose group,OD value,ALP activity,and the proportion of mineralized nodule area of medium-and high-dose groups were increased,JAK2 and p-STAT5/STAT5 were decreased(P<0.05);compared with medium-dose group,OD value,ALP activity,and the proportion of mineralized nodule area of high-dose group were increased,JAK2 and p-STAT5/STAT5 were decreased(P<0.05).Conclusion Icariin may promote the proliferation and osteogenic differentiation of BMSCs in rats with fracture trauma by inhibiting JAK2/STAT5 pathway.
作者
吴启弘
张永祥
余江
何立民
潘杰
杨颇
WU Qihong;ZHANG Yongxiang;YU Jiang;HE Limin;PAN Jie;YANG Po(Department of Traumatic Orthopedics,the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guizhou Province,Guiyang 550000,China)
出处
《中国医药导报》
CAS
2024年第7期23-26,共4页
China Medical Herald
基金
贵州省科技计划项目([2020]1Y369)。
关键词
淫羊藿苷
骨髓基质干细胞
增殖
成骨分化
Icariin
Bone marrow stromal cells
Proliferation
Osteogenic differentiation
