摘要
目的:研究仙茅苷对体外培养的大鼠骨髓基质细胞的增殖及成骨分化的影响。方法:将仙茅苷(25μg/mL、50μg/mL、100μg/mL,200μg/mL)作用于体外培养的大鼠骨髓基质细胞,CCK-8法检测仙茅苷对细胞增殖的影响,碱性磷酸酶(ALP)活性测定法进行ALP的表达情况的检测。结果:各浓度仙茅苷组均能促进大鼠骨髓基质细胞的增殖和碱性磷酸酶的表达(P<0.05),且在浓度为100μg/mL时作用最为显著。结论:仙茅苷对骨髓基质细胞的增殖有促进作用并能促进其成骨分化。
Objective:To observe the Effects of Curculigoside on proliferation and osteogenic differentiation of rat’s bone marrow stromal cells in vitro.Methods:Bone marrow stromal cells in vitro were cultured in medium contained different concentrations of Curculigoside(25μg/mL、50μg/mL、100μg/mL、200μg/mL).Cell Counting Kit-8 was used to test the proliferation of rat’s bone marrow stromal cells in vitro cultured with different concentrations of Curculigoside.ALP activity assay was used to observe the effects of osteogenic differentiation of rat’s bone marrow stromal cells in vitro cultured with Curculigoside.Results:Curculigoside enhanced the proliferation and ALP activity of rat’s bone marrow stromal cells in vitro(P<0.05),and 100μg/mL was the most effective one.Conclusion:Curculigoside could promote the proliferation and osteogenic differentiation of rat’s bone marrow stromal cells in vitro.
作者
苏佳雨
王锡明
赵刚
石金明
李超
柴傲然
SU Jiayu;WANG Ximing;ZHAO Gang;SHI Jinming;LI Chao;CHAI Aoran(Graduate School of Jiamusi University,Jiamusi 154007,China;General Hospital of Daqing Oil Field,Daqing163000,China;Second Affiliated Hospital of Jiamusi University,Jiamusi 154002,China)
出处
《黑龙江医药科学》
2024年第1期164-165,共2页
Heilongjiang Medicine and Pharmacy
关键词
仙茅苷
骨髓基质细胞
增殖
成骨分化
Curculigoside
bone marrow stromal cells
proliferation
osteogenic differentiation
