摘要
为开发针对陕西地区花椒主要病害的生防菌资源,对陕西省宝鸡市凤县花椒根际土壤微生物进行分离培养,并测定了其对花椒的4种靶标病原菌,即干腐病菌(Fusarium zanthoxyli)、黑胫病菌(Phytophthora citrophthora)、根腐病菌(F.solani)和落叶病菌(Marssonina sp.)的抑菌活性。结果显示:从花椒根际土壤中共分离到297个微生物菌株,其中105个为真菌,192个为细菌。通过初筛,获得了20个对3种及以上靶标病原菌均具有显著拮抗作用的细菌菌株,经形态学和16S rDNA序列分析,将其中7个鉴定为贝莱斯芽孢杆菌(Bacillus velezensis),5个鉴定为解淀粉芽孢杆菌(B.amyloliquefaciens),3个鉴定为伯克霍尔德氏菌属(Burkholderia)的不同种,2个鉴定为安徽黄杆菌(Flavobacterium anhuiense),3个为其他种。经复筛和验证,发现贝莱斯芽孢杆菌P87的生防潜力最强,可以作为花椒病害生物防治的备选菌种。
To develop biocontrol microbial resources for major diseases of Chinese prickly ash in Shaanxi Province,this study isolated and cultured microorganisms from the rhizosphere soil of Zanthoxylum bungeanum in Feng County,Baoji City,Shaanxi Province and determined their antifungal activity against four target pathogens of Zanthoxylum bungeanum,i.e.,Fusarium zanthoxyli,Phytophthora citrophthora,F.solani and Marssonina sp.The results showed that a total of 297 microbial strains were isolated from the rhizosphere soil of prickly ash,including 105 fungi and 192 bacteria.After initial screening,20 bacterial strains with significant antagonistic effects on three or more target pathogens were obtained.Through morphological and 16S rDNA sequence analysis,seven were identified as Bacillus velezensis,five as B.amyloliquefaciens,three as different species of Burkholderia genus,two as Flavobacterium anhuiense and three other species.After re-screening and verification,it was found that B.velezensis P87 had the strongest biocontrol potential,making it a viable alternative for biological control of Zanthoxylum bungeanum diseases.
作者
符晓
丁俊园
焦嘉卉
钟思雨
孙孟娇
付家佳
唐光辉
李培琴
FU Xiao;DING Junyuan;JIAO Jiahui;ZHONG Siyu;SUN Mengjiao;FU Jiajia;TANG Guanghui;LI Peiqin(Forestry College,Northwest A&F University,Key Laboratory of National Forestry and Grassland Administration on Management of Forest Bio-Disaster,Yangling,Shaanxi 712100,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2024年第2期222-230,244,共10页
Agricultural Research in the Arid Areas
基金
陕西省重点研发计划一般项目(2020NY-037,2023-YBNY-057)
西北农林科技大学科技创新与成果转化项目(TGZX2021-23)。
关键词
花椒
根际土壤
生防菌
筛选
鉴定
抑菌活性
Zanthoxylum bungeanum
rhizosphere soil
biocontrol bacteria
screening
identification
inhibitory activity
